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黄花败酱总皂苷的HPLC-Q-TOF MS成分分析 预览

Component analysis of total saponins from Patrinia scabiosaefolia Fisch by HPLC-Q-TOF MS
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摘要 目的采用高效液相色谱-高分辨飞行时间质谱(HPLC-Q-TOFMS)对黄花败酱总皂苷部位中的化学成分进行快速鉴别。方法采用ACQUITYUPLCTMBEHC18(2.1mm×50mm,1.7μm)色谱柱,以乙腈-(0.1%甲酸,10mmol甲酸铵)水溶液为流动相梯度洗脱,柱温:35℃,进样量:3μL,流速:0.3mL/min,采用ESI源在正离子模式下采集数据。根据化合物准确的相对分子质量及二级碎片离子信息,参考文献数据及与对照品对照,鉴定出黄花败酱总皂苷部位的主要化学成分。结果共鉴定出27个化学成分,其中三萜皂苷类成分10种,甾体皂苷2种,甾醇类3种,单萜类4种,有机酸类2种,糖苷类2种,黄酮类1种,其他类3种。结论HPLC-Q-TOFMS技术为鉴定黄花败酱有效部位化学成分提供了一种快速、高效的方法。 Objective To identify the chemical components of total saponins from Patrinia scabiosaefolia Fisch by high performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS).Methods The separation was performed on ACQUITY UPLCTM BEH C 18 column(2.1 mm×50 mm, 1.7 μm),and the gradient elution of acetonitrile-(0.1% formic acid,10 mmol ammonium formate) was used as mobile phase.The column temperature was 35 ℃,the flow rate was 0.3 mL/min and the injection volume was 3 μL.The data were collected by the positive and ion mode by using Q-TOF/MS and ESI source.The main chemical constituents of total saponins of Patrinia scabiosaefolia Fisch were identified according to the exact molecular mass,the cleavage fragments of MS/MS,the literature data and the reference control.Results Twenty seven chemical components were identified under the optimized liquid-mass spectrometry conditions,which were ten kinds of triterpenoid saponins,two kinds of steroidal saponins,three kinds of sterols,four kinds of monoterpenes,two kinds of organic acids,two kinds of glycosides,one kind of flavonoids and three kinds of other types.Conclusion The established HPLC-Q-TOF MS method provides a fast and efficient method for identifying the chemical constituents of the active part of the Patrinia scabiosaefolia Fisch.
作者 陈淑玲 王晓玉 钟艳梅 李卫东 韩亮 CHEN Shuling;WANG Xiaoyu;ZHONG Yanmei;LI Weidong;HAN Liang(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;School of Health,Guangdong Pharmaceutical University,Guangzhou 510006,China;Central Laboratory,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong Engineering Research Center for Light and Health,Guangzhou 510310,China)
出处 《广东药科大学学报》 CAS 2019年第2期199-203,共5页 Academic Journal of Guangdong College of Pharmacy
基金 国家自然科学基金青年基金项目(81403179,81703816).
关键词 黄花败酱 总皂苷 液相色谱-四极杆飞行时间质谱 成分分析 Patrinia scabiosaefolia Fisch total saponins HPLC-Q-TOF MS component analysis
作者简介 陈淑玲(1994—),女,2016级在读硕士研究生,Email:1562379995@qq.com;通信作者:韩亮(1980-),男,硕士研究生导师,副研究员,主要从事中药药效物质基础及作用机制研究,Email:hanliang72@126.com。
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