期刊文献+

盐胁迫下海水稻抗逆生理响应分析 预览

Physiological adversity resistance of sea rice to salinity stress
在线阅读 免费下载
收藏 分享 导出
摘要 以海水稻品种‘FL478’‘JX99’‘Pokkali’和盐敏感品种‘IR29’为材料,设置6 个土壤含盐量(0 g·kg^-1、1 g·kg^-1、2 g·kg^-1、3 g·kg^-1、4 g·kg^-1、5 g·kg^-1),在防雨大棚下进行盆栽试验,孕穗期测定水稻叶片的丙二醛含量、细胞膜透性、可溶性糖含量、脯氨酸含量,以及Δ^1-吡咯啉-5-羧酸合成酶(P5CS)、鸟氨酸-δ-氨基转移酶(δ-OAT)和超氧化物歧化酶(SOD)活性,旨在探讨盐胁迫下海水稻渗透调节物质、抗逆酶与海水稻耐盐性的关系,为海水稻耐盐基因的发掘和耐盐品种的选育提供理论依据。结果表明: 1)盐胁迫抑制了海水稻叶绿素的合成与积累,表现为水稻叶片的叶绿素含量随盐胁迫梯度的增加而减少,但其叶绿素含量显著高于盐敏感水稻品种。2)海水稻和盐敏感水稻的可溶性糖对盐胁迫的响应差异显著,其中土壤含盐量0~3 g·kg^-1 胁迫下,盐敏感水稻叶片的可溶性糖含量的增加显著高于海水稻,但是在土壤含盐量4~5 g·kg^-1 下,海水稻叶片的可溶性糖积累量显著高于盐敏感水稻品种。3)随盐胁迫加剧海水稻和盐敏感水稻叶片的丙二醛积累,造成细胞膜透性逐渐增大,但是海水稻品种丙二醛的积累量显著低于盐敏感对照,这表明海水稻叶片细胞膜损伤较小。4)盐胁迫下, 4 个水稻叶片的脯氨酸含量和P5CS、δ-OAT、SOD 活性随盐胁迫浓度增加均表现出先升高后降的趋势,海水稻和盐敏感水稻分别在3 g·kg?1、2 g·kg^-1 盐浓度达到峰值,而海水稻植株的游离脯氨酸积累量、P5CS、δ-OAT 和SOD 活性显著高于盐敏感水稻。5)盐胁迫下海水稻可溶性糖、脯氨酸和P5CS 之间呈极显著正的简单相关和偏相关性。因此,海水稻的抗盐性为生理性耐盐:在盐胁迫下,植株脯氨酸合成酶P5CS 和δ-OAT 均被激活,脯氨酸合成的两个途径(谷氨酸→脯氨酸和鸟氨酸→脯氨酸)同时增强,促使植株游离脯氨酸的快速积累� The sea rice varieties ‘FL478’‘JX99’‘Pokkali’ and salt-sensitive variety ‘IR29’ were used to determine malondialdehyde content, cell membrane permeability, soluble sugar and proline in rice leaf. We also investigated the activities of Δ^1-pyrroline-5-carboxylic acid synthetase (P5CS), ornithine-δ-aminotransferase (δ-OAT) and superoxide dismutase (SOD) under 0 g·kg^-1, 1 g·kg^-1, 2 g·kg^-1, 3 g·kg^-1, 4 g·kg^-1 and 5 g·kg^-1 of soil salt treatments at booting stage of potted rice under rainproof greenhouse conditions. This aim of the study was to investigate the relationship between osmotic regulators, stress resistance enzymes and salt tolerance of sea rice under salinity stress. The study also provided theoretical basis for the exploration of salt tolerance genes and breeding sea rice varieties with salt tolerance. The results were as follows: 1) salinity stress inhibited the synthesis and accumulation of chlorophyll in rice. Then chlorophyll content in rice leaf decreased with increasing salt stress concentration, and was significantly higher than that of salt-sensitive rice. 2) The response of soluble sugars of sea rice and salt-sensitive rice to salinity stress had significantly differences. The rise in soluble sugars of sea rice was less than that of salt-sensitive rice under 0~3 g·kg^-1 soil salt content, but the content of soluble sugars of sea rice was significantly higher than that of salt-sensitive rice under 4~5 g·kg^-1 soil salt content. 3) Malondialdehyde contents of sea rice and salt-sensitive varieties increased with increasing salt stress, resulting in increased cell membrane permeability. However, malondialdehyde content of sea rice was significantly lower than that of salt-sensitive rice. This implied that leaf membrane damage of sea rice was significantly less than that of salt-sensitive rice. 4) Proline content and P5CS,δ-OAT and SOD activities of rice leaf increased initially and then decreased with increasing salt stress. Proline content and P5CS,δ-OAT and SOD ac
作者 王旭明 赵夏夏 陈景阳 许江环 周柏霖 王盼盼 莫素 莫俊杰 谢平 周鸿凯 WANG Xuming;ZHAO Xiaxia;CHEN Jingyang;XU Jianghuan;ZHOU Bolin;WANG Panpan;MO Su;MO Junjie;XIE Ping;ZHOU Hongkai(College of Agriculture,Guangdong Ocean University,Zhanjiang 524088,China)
出处 《中国生态农业学报(中英文)》 CAS CSCD 北大核心 2019年第5期747-756,共10页 Chinese Journal of Eco-Agriculture
基金 国家自然科学基金项目(41073059) 广东省高等教育高校创新强校工程项目(GDOU2017052604, 2017KZDXM044) 广东省农业科技创新及推广项目(2018LM2173) 湛江市科技计划项目(2015A03015)资助.
关键词 海水稻 盐胁迫 抗逆生理 渗透调节 抗逆酶活性 Sea rice Salt stress Anti-stress physiology Osmotic regulation Resistance enzyme activity
作者简介 王旭明,主要从事作物抗逆生理研究, E-mail: m18793630087@163.com;通信作者:谢平,主要从事农业资源与环境方面的研究, E-mail: xiep168@163.com;通信作者:周鸿凯,主要从事作物学研究, E-mail: 897961801@qq.com.
  • 相关文献

参考文献21

二级参考文献317

共引文献306

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈