Objective:To investigate the protective effect of salidroside on induced osteoblasts in hypoxia environment and its mechanism. Methods:the mouse fibroblasts were separated and cultured by tissue block method. The transfected induced pluripotent stem cells were reprogrammed and the QT-PCR of Nanog, Oct-4, Rexl and Sox-2 were detected to the induced osteoblasts differentiated from the induced pluripotent stem cells and identified by alizarin red staining. The induced osteoblasts were divided into three groups:salidroside pre-conditioning group, normal oxygen group and hypoxia group. Annexin V-FITC/PI staining was used to detect the apoptosis rate, and Western-blot was used to detect VEGF, HIF-1 alpha. Results:The expression of endogenous gene Nanog, Oct-4, Rexl and Sox-2 of induced pluripotent stem cells was higher than that of mouse fibroblasts cells. The result is Nanog (783±42 vs. 308±23), Oct-4 (774±37 vs. 319±31), Rexl (742±28 vs. 382±31), Sox-2 (831±31 vs. 394±12). Alizarin red staining showed that the induced osteoblasts cells had positive staining results. The apoptotic rate of salidroside group was significantly lower than that of hypoxia group [(35.65±3.92)% vs.(95.20±1.03)%]. There was significant difference between the two groups (P<0.05). The expression of HIF-1a and VEGF in salidroside group was higher than that in hypoxic group (0.93±0.05 vs. 0.81±0.02, 0.95±0.03 vs. 0.79±0.04, P<0.05). Conclusion:Salidroside has protective effect on hypoxic environment of osteoblasts.
Journal of Wenzhou Medical College
induced pluripotent stem cells