Objective To screen out the pathogenic genes of psoriatic arthritis (PsA) and to analyze the pathogenic genes by bioinformatics. Methods Gene expression profile GSE61281 was downloaded from NCBI of American Bioinformation Technology Center. Peripheral blood samples of PsA patients and control group were analyzed by R programming language to identify the differentially expressed genes (DEGs). Then, the pathogenic genes of PsA were screened out followed by GLAD4U data ToppGene database. In addition, DAVID and KOBAS3.0 online tools were used to analyze the enrichment of pathogenic genes, and the PPI network of PsA pathogenic genes was constructed by String database. Finally, the key genes, main biological processes, and signaling pathways in the pathogenesis of PsA were screened. Results The pathogenic genes closely related to the pathogenesis of PsA were obtained. CXCL10, LYN, JAK1, CARD11 and ANXA1 were highly correlated with PsA (with large scores and significant differences). The results of GO enrichment analysis showed that there were many genes significantly enriched in the immune response, inflammatory response, innate immune response, signal transduction, and response to lipopolysaccharide and other biological processes. KEGG analysis showed that the mainly enriched pathways were the interactions between cytokines and cytokine receptors, and inflammatory bowel disease, etc. There were interactions between the selected PsA pathogenic genes and TNF, IL1B, IL13, IL17A, CCL2, and IL23R. Conclusions The genes highly related to the pathogenesis of PsA are CXCL10, LYN, JAK1, CARD11, and ANXA1, which are involved in the immune response, inflammatory response, innate immune response, signal transduction, and response to lipopolysaccharide and other biological processes. They may affect the interactions between cytokines and cytokine receptors, inflammatory bowel disease, and other pathways, and have interactions with TNF, IL1B, IL13, IL17A, CCL2, and IL23R.
Shandong Medical Journal
oligonucleotide array sequence analysis