期刊文献+

红景天苷对急性脑出血大鼠神经功能缺损评分与脑含水量的影响及其作用机制研究 预览

Effect of Salidroside on Neurological Deficit Score and Brain Water Content in Rats with Acute Intracerebral Hemorrhage and Its Mechanism
在线阅读 免费下载
收藏 分享 导出
摘要 目的探讨红景天苷对脑出血(ICH)大鼠神经功能缺损评分与脑含水量的影响,并探讨其可能的机制。方法将75只大鼠随机分为5组,假手术组(Sham)、脑出血组(ICH)、药物低剂量组(Sal-L)、药物中剂量组(Sal-M)和药物高剂量组(Sal-H),每组15只,建立脑出血大鼠模型,药物低剂量组、药物中剂量组和药物高剂量组分别给予红景天苷灌胃10mg/kg、20mg/kg和40mg/kg,每日1次,共给药10d。对各组大鼠进行神经功能缺损评分;称重法计算脑含水量;商业试剂盒检测脑组织中髓过氧化物酶(MPO)活性;免疫印记法与RT-PCR分别检测脑血肿组织周围缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)蛋白与mRNA表达。结果采用红景天苷处理后,脑出血大鼠的神经功能缺损评分明显低于无药物处理的大鼠,脑含水量明显下降,脑组织中MPO活性明显下降;脑血肿组织周围的HIF-1α和VEGF蛋白与mRNA表达明显上调。结论红景天苷具有保护急性脑出血大鼠脑组织的作用,可能与红景天苷能够上调脑血肿组织周围HIF-1α和VEGF因子表达有关。 Objective To investigate the effect of salidroside on intracerebral hemorrhage (ICH) and to explore the possible mechanism. Methods Seventy-five rats were randomly divided into 5 groups:sham group,ICH group,low dosage treatment group,medium dosage treatment group and high dosage treatment group. After establishing ICH model,rats were received 10mg/kg,20mg/kg or 40mg/kg Salidroside by gavage once a day for 10 days. The neuronal function impairment of rats was scored and the brain water content was measured. The myeloperoxidase (MPO) activity in brain was determined. Moreover,the protein and mRNA expression of hypoxia inducible factor 1α(HIF-1α) and vascular endothelial growth factor (VEGF) in brain hematoma tissues were detected by western blotting and RT-PCR,respectively. Results After Salidroside treatment,the score of neuronal function impairment was lower than ICH rats without treatment.In addition,the brain water content and MPO activity were significantly decreased after Salidroside administration,whereas the protein and mRNA expression of HIF-1αand VEGF in brain hematoma tissues were increased. Conclusion Salidroside can exert protective effects on brain tissues in ICH rats. These effects may be through increasing HIF-1αand VEGF expression in brain hematoma tissues.
作者 赵辉 白玉彦 温桂莲 赵艺 ZHAO Hui;BAI Yuyan;WEN Guilian;ZHAO Yi(Nanyang City Centeral Hospital,Nanyang 473000,Henan,China)
机构地区 南阳市中心医院
出处 《中西医结合心脑血管病杂志》 2019年第3期368-371,共4页 Chinese Journal of Integrative Medicine on Cardio-/Cerebrovascular Disease
关键词 脑出血 红景天苷 缺氧诱导因子-1Α 血管内皮生长因子 神经功能缺损评分 脑含水量 intracerebral hemorrhage salidroside hypoxia inducible factor-1α vascular endothelial growth factor neurological deficit score brain water content
作者简介 赵辉,E-mail:3163067814@qq.com
  • 相关文献

参考文献5

二级参考文献88

共引文献70

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈
新型冠状病毒肺炎防控与诊疗专栏