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中药补肾活血方提取物促进SD大鼠骨髓间充质干细胞增殖作用的最佳浓度 预览

Bushen Huoxue Recipe extract at the optimal concentration promotes the proliferation of bone marrow mesenchymal stem cells of Sprague-Dawley rats
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摘要 背景:目前补肾活血方干预骨髓间充质干细胞增殖分化的实验中,其最佳影响质量浓度是多少,尚不明确。目的:探讨补肾活血方提取物诱导骨髓间充质干细胞增殖分化的最佳质量浓度和最佳时间。方法:采用贴壁筛选培养法分离纯化SD大鼠骨髓间充质干细胞,取第3代细胞用于实验。将补肾活血方提取物按质量浓度梯度(0,10,20,40,100,120,150,200,300,400,500,600mg/L)干预第3代骨髓间充质干细胞,诱导时间分别为1,3,5,7d。荧光倒置显微镜观察补肾活血方提取物干预后骨髓间充质干细胞的生长情况。用CCK-8法检测不同质量浓度补肾活血方提取物对骨髓间充质干细胞的毒性,筛选最佳药物质量浓度和作用时间。结果与结论:与0mg/L组比较,第5,7天时,100,120,150mg/L补肾活血方提取物可促进骨髓间充质干细胞增殖(P<0.05);在第7天时,150mg/L补肾活血方提取物促增殖作用最强(P<0.01);药物质量浓度在150-200mg/L时,增殖率下降明显;随着实验时间的延长,大于200mg/L时,骨髓间充质干细胞增殖不明显。 BACKGROUND:It is unclear what is the optimal concentration of Bushen Huoxue Recipe to promote the proliferation and differentiation of bone marrow mesenchymal stem cells.OBJECTIVE:To investigate the optimal concentration and induction time of Bushen Huoxue Recipe extract to induce the proliferation and differentiation of bone marrow mesenchymal stem cells.METHODS:Bone marrow mesenchymal stem cells of Sprague-Dawley rats were isolated and purified by adherent screening culture.Then,the bone marrow mesenchymal stem cells of3rd generation were cultured with the extracts of Bushen Huoxue Recipe at different concentration(0,10,20,40,100,120,150,200,300,400,500and600mg/L).The induction time was1,3,5and7days,respectively.The growth of bone marrow mesenchymal stem cells induced by Bushen Huoxue Recipe extracts was observed by fluorescent inverted microscope.The toxicity of different concentration solutions to bone marrow mesenchymal stem cells was detected by cell counting kit-8for screening the optimal drug concentration and induction time.RESULTS AND CONCLUSION:Compared with the0mg/L group,100,120and150mg/L extracts from Bushen Huoxue Recipe significantly promoted the proliferation of bone marrow mesenchymal stem cells at the5th and7th days(P<0.05).The150mg/L Bushen Huoxue Recipe extract showed the strongest proliferative effect(P<0.01),while the proliferation rate decreased significantly,when the mass concentration of Bushen Huoxue Recipe extract was150-200mg/L.With the extension of the induction time,the proliferation of bone marrow mesenchymal stem cells showed no obvious changes after intervention with Bushen Huoxue Recipe at a concentration of more than200mg/L.
作者 卫荣 温欣 王莉 魏志勇 Wei Rong;Wen Xin;Wang Li;Wei Zhiyong(Xinjiang Academy of Traditional Chinese Medicine, Urumqi830000, Xinjiang Uygur Autonomous Region, China;the People’s Hospital of Suzhou New District, Suzhou215000, Jiangsu Province, China;Kuitun Hospital of Xinjiang Production and Construction Corps, Kuitun 833200,Xinjiang Uygur Autonomous Region, China)
出处 《中国组织工程研究》 CAS 北大核心 2019年第5期691-696,共6页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 国家自然科学基金项目(81460676),项目负责人:卫荣 国家第55批博士后基金项目,项目负责人:卫荣.
关键词 骨髓间充质干细胞 补肾活血方 细胞毒性 干细胞 国家自然科学基金 骨髓 间质干细胞 中草药 细胞增殖 组织工程 Bone Marrow Mesenchymal Stem Cells Drugs,Chinese Herbal Cell Proliferation Tissue Engineering
作者简介 卫荣,女,1968年生,陕西省韩城市人,汉族,2009年新疆医科大学毕业,博士,主任医师,教授,主要从事中西医结合治疗风湿病的临床与基础研究;通讯作者:魏志勇,副主任医师,新疆生产建设兵团奎屯中医院,新疆维吾尔自治区奎屯市833200
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