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调节性T细胞在结直肠癌患者手术及化疗前后外周血中的变化及意义 预览

Changes and significance of regulatory T cells in peripheral blood of patients with colorectal cancer before and after surgery and chemotherapy
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摘要 目的 通过观察结直肠癌(colorectal cancer,CRC)患者手术及化疗前后外周血中调节性T细胞(regulatory T cell,Treg),探讨Treg在肿瘤免疫中的作用及相关机制,同时分析手术及化疗对其变化的影响。方法 临床选取15例CRC患者,均参与CRC根治性切除,且术后2周行“5-Fu”为基础的三周方案进行化疗。分别采集患者在术前1天、术后2周、第2次化疗结束后2周的外周血,用流式细胞术检测CD4^+CD25^+Foxp3^+Treg在外周血CD4^+T细胞中的比例,并以14例健康者外周血作为对照,对所得结果用统计软件进行分析。结果 CRC患者外周血中CD4^+CD25^+Foxp3^+Treg占CD4^+T细胞的比例为(6.92±2.12)%,显著高于健康组(4.66±0.92)%,差异有统计学意义(P<0.05);经手术及化疗,患者外周血中CD4^+CD25^+Foxp3^+Treg较术前无显著变化(P>0.05)。结论 CRC患者外周血中Treg可能参与了肿瘤免疫逃逸,手术及化疗后机体免疫功能可能仍受抑制,因此是进行生物治疗的最佳窗口期。 Objective To understand the role of regulatory T cells(Tregs)in tumor immunology and the mechanism of Tregs therein and to evaluate the effects of surgery and chemotherapy on the changes in Tregs by mapping the changes of Tregs in the peripheral blood of patients with colorectal cancer(CRC)before and after surgery as well as before and after chemotherapy.Methods Firstly,15 CRC patients were selected as the subjects of study.All subjects were then subjected to radical resection and two weeks after surgery subjected to chemotherapy based on a 5-Fu-based 3-week regimen.Samples of peripheral blood was collected from the patients one day before surgery,two weeks after surgery and two weeks after the second session of chemo.The samples were then subjected to flow cytometry for detection of the proportions of CD4+CD25+Foxp3+Tregs in CD4+T cells therein.The Results from the detection were then compared with those from the samples of 14 healthy subjects chosen as the control group by means of a statistical analysis software.Results The proportion of CD4+CD25+Foxp3+Tregs in CD4+T cells in peripheral blood of CRC patients was(6.92±2.12)%,which was significantly higher than that,(4.66±0.92)%,in the control group.The difference was statistically significant(P<0.05).No significant difference was observed between the CD4+CD25+Foxp3+Tregs in peripheral blood of patients after surgery and chemotherapy and that before surgery and chemotherapy(P>0.05).Conclusions Tregs in peripheral blood of CRC patients might play a role in tumor immune escape.The immune performance could remain suppressed following surgery and chemotherapy,which engender the optimal opportunity for biotherapy.
作者 沈军权 张汝一 甄运寰 修瑾 SHEN Jun-quan;ZHANG Ru-yi;ZHEN Yun-huan;XIU Jin(Department of Anorectal Surgery,The People’s Hospital of Yuyao,Yuyao 315400;Department of Anorectal Surgery, The Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China)
出处 《健康研究》 CAS 2018年第6期661-664,671共5页 Health Research
关键词 调节性T细胞 结直肠癌 手术 化疗 regulatory T cells colorectal cancer surgery chemotherapy
作者简介 沈军权(1982-),男,浙江余姚人,硕士,主治医师;通讯作者:张汝一(1964-),男,贵州贵阳人,本科,主任医师。
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