期刊文献+

硫辛酸对脂多糖诱导帕金森病细胞模型的保护作用及机制 预览

Protective effects and the mechanism of lipoic acid on cell model of Parkinson’s disease induced by lipopolysaccharide
在线阅读 下载PDF
收藏 分享 导出
摘要 背景:近年越来越多的研究证实神经免疫和炎症反应参与了帕金森病的发病,硫辛酸目前被证实具有抗炎和神经保护作用。目的:探讨硫辛酸对脂多糖诱导PC12细胞制备的帕金森病细胞模型的保护作用及相关可能机制。方法:将PC12细胞分为正常细胞组、脂多糖-帕金森病组和硫辛酸+脂多糖-帕金森病组。硫辛酸预处理PC12细胞2h后,再用脂多糖干预PC12细胞。用CCK8法筛选最佳帕金森细胞模型制备的脂多糖浓度以及硫辛酸最佳干预浓度;细胞免疫组织化学法检测各组细胞中酪氨酸羟化酶的表达;ELISA法检测各组中细胞上清液中白细胞介素6和肿瘤坏死因子α水平;Westernblot检测各组细胞中NF-κBp65、酪氨酸羟化酶的蛋白表达情况。结果与结论:①建造帕金森细胞模型最佳脂多糖的质量浓度为600μg/L,最佳硫辛酸干预浓度为25μmol/L;②硫辛酸干预后可以促进酪氨酸羟化酶阳性细胞表达,减少帕金森病细胞模型的炎症因子释放,并且可以抑制NF-κBp65的产生,提高酪氨酸羟化酶含量;③结果提示,硫辛酸对帕金森病细胞模型的作用可能通过抑制NF-κB信号通路,减轻炎症状态,从而起到对神经元的保护作用。 BACKGROUND:Neuroimmune and inflammatory reaction have been shown to participate in the occurrence of Parkinson’s disease.Lipoic acid possesses anti-inflammatory and neuroprotective roles.OBJECTIVE:To investigate the protective effects of lipoic acid on PC12 cell models of Parkinson’s disease induced by lipopolysaccharide and the underlying mechanism.METHODS:PC12 cells were divided into blank control,model(Parkinson’s disease model induced by lipopolysaccharide)and treatment(Parkinson’s disease model treated by lipoic acid)groups.PC12 cells were pretreated with lipoic acid for 2 hours,and then stimulated with lipopolysaccharide.The optimal concentration of lipopolysaccharide and lipoic acid was determined by cell counting-kit 8 assay.The expression of tyrosine hydroxylase was detected by immunohistochemistry.The levels of tumor necrosis factorαand interleukin 6 were detected by ELISA kit.The protein expression levels of nuclear factor-κBp65 and tyrosine hydroxylase were detected by western blot assay.RESULTS AND CONCLUSION:The optimal concentration of lipopolysaccharide was 600μg/L for constructing cell model of Parkinson disease,and 25μmol/L lipoic acid was the optimal intervention concentration.Lipoic acid could increase the number of tyrosine hydroxylase positive cells,reduce the release of inflammatory factors in cell model of Parkinson’s disease,inhibit the production of nuclear factor-κBp65 and increase tyrosine hydroxylase content.Therefore,lipoic acid exhibits its effects on cell model of Parkinson’s disease through inhibiting the nuclear factor-κB signaling pathway and reducing inflammation,thereby exerting neuroprotection.
作者 高华 李艳霞 王丹 杨新玲 Gao Hua;Li Yanxia;Wang Dan;Yang Xinling(Department of Neurology,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830063,Xinjiang Uygur Autonomous Region,China;Department of Neurology,the Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China)
出处 《中国组织工程研究》 CAS 北大核心 2019年第3期447-452,共6页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 国家自然科学基金项目(U1503222),项目负责人:杨新玲。
关键词 硫辛酸 帕金森病 脂多糖 NF-κB PC12细胞 白细胞介素6 肿瘤坏死因子Α 组织构建 硫辛酸 帕金森病 脂多糖类 白细胞介素6 肿瘤坏死因子Α 组织工程 Thioctic Acid Parkinson Disease Lipopolysaccharides Interleukin-6 Tumor Necrosis Factor-alpha Tissue Engineering
作者简介 高华,女,1979年生,新疆维吾尔自治区伊犁巩留县人,汉族,2018年新疆医科大学毕业,博士,主治医师,主要从事帕金森病的研究;通讯作者:杨新玲,博士,主任医师,新疆医科大学第二附属医院神经内科,新疆维吾尔自治区乌鲁木齐市830063。
  • 相关文献

参考文献4

二级参考文献35

  • 1McGeer EG,Klegeris A,McGeer PL,et al.Inflammation,the complement system and the diseases of aging[J].Neurobiol Aging,2005,26(Suppl 1):94-97. 被引量:1
  • 2Wyss-Coray T.Inflammation in Alzheimer disease:driving force,bystander or beneficial response?[J].Nat Med,2006,12(9):1005-1015. 被引量:1
  • 3Kopnisky KL,Bao J,Lin YW.Neurobiology of HIV,psychiatric and substance abuse comorbidity research:Workshop report[J].Brain Behav Immun,2007,21(4):428-441. 被引量:1
  • 4Bruck W,Stadelmann C.Inflammation and degeneration in multiple sclerosis[J].Neurol Sci,2003,24(Suppl 5):S265-S267. 被引量:1
  • 5Barouch FC,Miller JW.The role of inflammation and infection in age-related macular degeneration[J].Int Ophthalmol Clin,2007,47(2):185-197. 被引量:1
  • 6Daniel S,Limson JL,Dairam A,et al.Through metal binding,curcumin protects against lead-and cadmium-induced lipid peroxidation in rat brain homogenates and against lead-induced tissue damage in rat brain[J].J Inorg Biochem,2004,98(2):266-275. 被引量:1
  • 7Hellstrom IC,Danik M,Luheshi GN,et al.Chronic LPS exposure produces changes in intrinsic membrane properties and a sustained IL-b-dependent increase in GABAergic inhibition in hippocampal CA1 pyramidal neurons[J].Hippocampus,2005,15(5):656-664. 被引量:1
  • 8Bickler PE,Fahlman CS,Gray J,et al.Inositol 1,4,5-triphosphate receptors and NAD(P)H mediate Ca^2+ signaling required for hypoxic preconditioning of hippocampal neurons[J].Neuroscience,2009,160 (1):51-60. 被引量:1
  • 9Smith AR, Shenvi SV, Widlansky M, et al. Lipoie acid as a potential therapy for chronic diseases associated with oxidative stress [ J ]. Curr Med Chem, 2004, 11 ( 9 ) : 1135-1146. 被引量:1
  • 10Zaitone SA, Abo-Elmatty DM, Shaalan AA. Acetyl-L- carnitine and α-lipoic acid affect rotenone-induced damage in nigral dopaminergic neurons of rat brain, implication for Parkinson's disease therapy [ J ]. Pharmacol Biochem Be- hay, 2012, 100(3) :347-360. 被引量:1

共引文献21

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈