目的探索肥胖状态下牙周炎对模型动物的内脏重量和系数的影响。方法将64只C57BL/6J小鼠随机分为高脂饲料（HFD）组（n=36）和低脂饲料（LFD）（n=28）组,HFD组诱导肥胖至处死,LFD组作为正常体重对照。诱导16周,两饲料组均再分为牙周炎（P）组和对照（C）组,P组用长有牙龈卟啉单胞菌的丝线分别结扎5、10 d诱导牙周炎,C组做假性结扎。结扎后处死小鼠,分离内脏称重并计算内脏系数。结果与LFD组相比,HFD组上调内脏脂肪和脾的重量、系数（P〈0.05）,上调肝、肾重量（P〈0.05）,下调肝系数和肾系数（P〈0.01）。HFD组中,P组相对于C组下调脾重量和系数（P〈0.05）,而结扎10 d相对于结扎5 d上调脾重量和系数（P〈0.05）。结论牙周炎可影响肥胖模型动物内脏（特别是脾脏）的大体形态,该现象在肥胖与牙周炎的相互关系中可能具有免疫代谢学层面的病理意义。
Objective This study aimed to explore the impacts of periodontitis on the visceral weight and weight percentage of obese animal models. Methods A total of 64 C57 BL/6 J mice were divided into the following diet groups： high-fat diet（HFD） group（n=36）, which was fed with high-fat diet to induce obesity, and low-fat diet（LFD） group（n=28）, which was fed with low-fat diet as the control. After 16 weeks on diet, each diet group was divided into periodontitis（P） and control（C） groups. The P groups were induced for periodontitis by ligation with Porphyromonas gingivalis-adhered silk for 5 or 10 days, and the C groups were sham-ligated as the control. Visceral organs were resected and weighed. The organ weight percentage was calculated. Results Compared with the LFD group, the HFD group significantly upregulated the weight and weight percentage of visceral adipose tissue and spleen（P〈0.05）, upregulated the weight of liver and kidney（P〈0.05）, and downregulated the weight percentage of liver and kidney（P〈0.01）. In the HFD group, the weight and weight percentage of spleen were downregulated in the P group（P〈0.05）, but were upregulated in the 10-day group compared with the 5-day group（P〈0.05）. Conclusion Periodontitis can affect the general morphology of the viscera（especially spleen） in obese animal models. Pathological indications in terms of immunometabo-lism might be present in the correlation between obesity and periodontitis.
West China Journal of Stomatology