期刊文献+

心脏停搏液中不同浓度钾离子和镁离子对猪冠状动脉平滑肌功能的影响

Effects of different concentrations of potassium and magnesium in cardiac arrest solution on the function of porcine coronary artery smooth muscle
分享 导出
摘要 目的观察心脏停搏液中不同浓度钾离子和镁离子对猪冠状动脉平滑肌功能的影响。方法选择健康瑞典家猪24头,随机分成四组,每组6头。取出冠状动脉前降支远端1/3,将其截成长度1 mm的血管环,进行器官浴槽实验。第一组向器官浴槽内加入16 mmol/L KCl-Krebs液,累积增加镁离子浓度至0、1.2、2、4、6、8、10、12、14、16、18、20 mmol/L,记录每个镁离子浓度下血管收缩力。第二组加入23 mmol/L KCl-Krebs液,第三组加入30 mmol/L KCl-Krebs液,第四组加入40 mmol/L KCl-Krebs液,镁离子浓度均与第一组相同。评价四组冠状动脉平滑肌收缩力的变化。结果血管收缩力随着钾离子浓度升高,收缩力曲线明显上移。各组中钾离子浓度不变时,随着镁离子浓度的增加,收缩力逐渐降低。以正常镁离子浓度(1.2 mmol/L)时,钾离子诱导的收缩力为100%,收缩力被拮抗80%时,四组的镁离子浓度分别为8、10、10、12 mmol/L。结论心脏晶体高钾停搏液中钾离子浓度为16~23 mmol/L,镁离子浓度为8~12 mmol/L时,对离体猪冠状动脉血管平滑肌功能影响较小,有利于停搏液的充分灌注和心肌保护。 Objective To study the effect of different concentrations of potassium and magnesi- um in cardiac arrest solution on the function of porcine coronary artery smooth muscle. Methods Twen- ty-four healthy Swedish domestic pigs were randomly divided into 4 groups, with 6 pigs in each group. The distal 1/3 of the left anterior descending coronary artery was dissected and cut into 1 mm long seg- ments and then transferred into organ bath. In the first group, 16 mmol/L KC1-Krebs solution with ac- cumulatively increasing concentrations of magnesium (0, 1.2, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20 mmol/L) was added into the organ bath. The vasoconstrictor force of each magnesium ion concentration was recor- ded. In the second group, 23 mmoL/L KC1-Krebs solution was added, and in the third group, 30 mmol/L KC1-Krebs solution was added, and in the fourth group, 40 mmol/L KC1-Krebs solution was added. The magnesium ion concentration was the same as that of the first group. The changes in the contractility of the coronary artery smooth muscle were evaluated in the four groups. Results The contractions of the coronary artery were significantly higher when the concentrations of potassium were higher. When the concentrations of potassium were constant, the contractions decreased as the concentrations of magnesium increased. If the contractions induced with KC1-Krebs solutions with 1.2 mmol/L, and the potassium-in-duced contractile force was 100%. When the contractile force was antagonized to 80%, the magnesium concentrations in the four groups respectively were 8, 10, 10, and 12 mmol/L. Conclusions The po- tassium concentration in the cardiac potassium high potassium cardioplegic solution is 16 -23 mmol/L and the magnesium ion concentration is 8 - 12 mmol/L. It has little effect on the isolated porcine coro- nary vascular smooth muscle function, which is beneficial to the full perfusion of the cardioplegia and the protection of the myocardium.
作者 陈力维 张伟华 臧素华 马宁 罗鸿 秦广启 Stig Steen 乔晨晖 Ma Ning, Luo Hong, Qin Guangqi, Stig Steen, Qiao Chenhui (Department of Cardiovascular Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China ; Department of Cardiothoracic Surgery, Lund University Hospital, Lund 22594, Sweden )
出处 《中国实用医刊》 2018年第10期6-9,共4页 Chinese Journal of Practical Medicine
基金 河南省科技厅科技攻关项目(142102310346) 河南省医学科技攻关计划普通项目(201602064)
关键词 心脏停搏液 冠状动脉 平滑肌 钾离子 镁离子 Cardiac arrest fluid Coronary artery Smooth muscle Potassium ion Magnesium ion
作者简介 通信作者:乔晨晖,Email:qchenhui@hotmail.com
  • 相关文献

参考文献2

二级参考文献22

  • 1熊利华,苏艳玲,华平,陈炬,潘越江,张惠忠,张华,黄石辉.不停跳心内直视手术的临床研究[J].中山大学学报:医学科学版,2004,25(B07):221-223. 被引量:9
  • 2范全心,李德才,王安彪,杜亮,訾捷,张文龙,邹承伟.HTK液与含血停搏液心肌保护效果比较[J].山东医药,2006,46(6):3-5. 被引量:7
  • 3熊利华,黄景彬,魏益平,李佳,祝曙光,华平,王铭辉,张华.不停跳心内直视手术对心肌细胞凋亡的影响[J].中山大学学报:医学科学版,2007,28(5):562-564. 被引量:4
  • 4Demmy T L, Biddle J S, Bennett L E, et al. Organ preser- vation solutions in heart transplantation-patterns of usage and related survival [J]. Transplantation, 1997, 63 (2): 262-269. 被引量:1
  • 5Wang D, Savage C, Xiao S, et al. St Thomas Hospital Cardioplegia #2 fails to suppress slow reaction pacemaker cell electrical activity[ J]. J Surg Res, 2004,120( 1 ):56-63. 被引量:1
  • 6Liu J, Feng Z, Zhao J, et al. The myocardial protection of HTK cardioplegic solution on the long-term ischemic period in pediatric heart surgery [J]. ASAIO J, 2008, 54 (5):470-473. 被引量:1
  • 7Li J A, Liu Y L, Liu J P, et al. Pulmonary artery perfu- sion with HTK solution prevents lung injury in infants af- ter cardiopulmonary bypass [J]. Chin Med J (Engl) , 2010, 123( 19):2645-2650. 被引量:1
  • 8Kober I M, Obermayr R P, Brull T, et al. Comparison of the solutions of Bretschneider, St. Thomas' Hospital and the National Institutes of Health for cardioplegic protec- tion during moderate hypothermic arrest [J]. Eur Surg Res, 1998,30(4):243-251. 被引量:1
  • 9Rauen U, Klempt S, de Groot H. Histidine-induced injury to cultured liver cells, effects of histidine derivatives and of iron chelators [J]. Cell Mol Life Sci, 2007, 64 (2): 192-205. 被引量:1
  • 10Wu K, Turk T R, Rauen U, et al. Prolonged cold storage using a new histidine-tryptophan-ketoglutarate-based pres- ervation solution in isogeneic cardiac mouse grafts [J]. Eur Heart J,2011,32(4):509-516. 被引量:1

共引文献1

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈