期刊文献+

蝙蝠葛苏林碱对HEK293细胞中L-型钙通道CaV 1.2的作用 被引量:1

Effects of Daurisoline on L-Type Calcium Channel Cav1. 2 Expressed in HEK293 Cells
收藏 分享 导出
摘要 目的:探讨蝙蝠葛苏林碱(DS)对人胚肾细胞(HEK293)上表达的L-型钙通道Cav1.2的作用。方法:采用脂质体转染法,将L-型钙通道Cav1.2蛋白基因转染到HEK293细胞上,全细胞膜片钳技术记录L-型钙电流ICav1.2,观察DS对L-型钙通道电生理特性的影响。结果:DS在1,3,10μmol·L-1浓度范围内呈浓度依赖性抑制ICav1.2电流,其抑制率分别为(14.68±4.02)%、(32.37±6.63)%和(59.63±5.23)%;3μmol·L-1的DS对ICav1.2的抑制率与相同浓度的伊拉地平比较,其抑制强度约为伊拉地平的40%。结论:DS可浓度依赖性抑制HEK293细胞上表达的Cav1.2,其作用强度比特异性L-型钙通道阻断药伊拉地平要弱。 Objective: To investigate the effects of daurinsoline (DS) on L-type calcium channel Carl.2 expressed in HEK293 cells. Methods: Cav1. 2 was transferred into HEK293 cells using Lipofectamine 2000, and the effects of DS on Cav1. 2 currents (ICav1.2) were analyzed by whole-cell patch clamp techniques. Results: DS at 1, 3 and 10 μmol · L-1could inhibit the ICav1 2in HEK293 cells in a dose-dependent manner. The inhibitory rate was (14.68 ± 4.02) %, (32.37 ± 6.63) % and (59.63 ± 5.23) %, respectively. The inhibitory rate of DS at 3 μmol · L-1 was 40 % of that of 3 μmol· L-1 isradipine (a L-type calcium channel blocker). Conclusion: DS can inhibit the Icavl.2 in HEK293 cells in a dose-dependent manner and the inhibition of DS is weaker than that of isradipine.
作者 胡德凤 丁杰 郭莲军 Hu Defeng1, Ding Jie2, Guo Lianjun2(1. Yiling Hospital, Hubei Yichang 443100, China; 2. Department of Pharmacology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology)
出处 《中国药师》 CAS 2018年第5期776-778,共3页 China Pharmacist
关键词 HEK293细胞 L-型钙通道Cav 1.2 蝙蝠葛苏林碱 伊拉地平 电生理特性 HEK293 cells L-Type calcium channel Cav1.2 Daurisoline Isradipine Electrophysiologic characteristic
作者简介 通讯作者:郭莲军Tel:18971693375E-mail:gljy2008@126.com.
  • 相关文献
投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈
新型冠状病毒肺炎防控与诊疗专栏