为了研究甜樱桃（Prunus avium）成花调控关键基因在不同砧穗组合中表达差异,以甜樱桃‘艳阳’（Sunburst）为接穗,‘ZY-1’（P.cerasus）、马哈利（P.mahaleb）和‘兰丁2号’（P.avium × P.pseudocerasus）为砧木的嫁接组合为试材,调查统计4年干龄不同砧穗组合的总花芽量及开花情况;克隆甜樱桃成花调控网络中EARLY FLOWERING 3（ELF3）、CONSTANS（CO）、FLOWERING LOCUS T（FT）等9个关键作用基因,并利用实时荧光定量PCR对不同砧穗组合接穗的幼叶、叶芽、成龄叶、花芽、叶芽附近叶片叶柄及花芽附近叶片叶柄中这些基因的表达量鉴定。结果显示,艳阳/ZY-1、艳阳/马哈利和艳阳/兰丁2号组合的平均单株花芽量分别为279、288和317朵,不存在明显差异,但不同组合的花期却明显不同,当艳阳/马哈利有72%花芽处于开放期时,艳阳/兰丁2号的花开放比例只有7%,艳阳/ZY-1的花开放比例为49%;此外,PaELF3、PaCO、PaFT等9个成花关键基因,在同一时期不同砧穗组合的接穗组织中表达量存在差异,其中PaAP1在不同砧穗组合的花芽中表达模式与花期规律一致,表明PaAP1与樱桃花期调控密切相关。
In order to investigate the differences of flowering-related gene expression patterns among different scion-stock combinations in cherry,here a cultivar namely‘Sunburst＇of sweet cherry was used as scion and‘ZY-1＇（Prunus cerasus）,P. mahaleb and‘Landing 2＇（P. avium × P. pseudocerasus）were used as rootstock,respectively. Then the quantities of flower buds and the flowering stage in these different rootstocks were investigated in all four-year-old grafted combinations. In addition, nine flowering-related genes that function mainly in flowering such as EARLY FLOWERING 3（ELF3）,CONSTANS（CO）,FLOWERING LOCUS T（FT）and so on from sweet cherry were isolated and identified. And quantitative PCR was recruited to analyze the relative expression level of these genes in young leaves,leaf buds,mature leaves,flower buds,petiole close to the leaf bud and petiole close to the flower bud of different grafting combinations. The results showed that the average quantities of flower buds of Sunburst/ZY-1,Sunburst/Mahaleb and Sunburst/Landing 2 were 279,288 and 317,respectively,indicating that there is no significant difference between these combinations. However,only 7% flower buds were full blooming in Sunburst/Landing 2 when 72% in Sunburst/Mahaleb,and 49% in Sunburst/ZY-1 at same times,suggesting that the blooming time of Sunburst/Landing 2 is obviously later than the other two. Furthermore,full-length of nine flowering-related genes were cloned and named as PaELF3,PaCO,PaFT and so on. Quantitative RT-PCR results indicated that nine flowering-related genes exhibited different expression patterns in different tissues. Among them,the expression pattern of PaAP1 in flower buds was co-related to blossom time,proving that PaAP1 is most closely to the cherry blossom time. This study provides an important new reference on the mechanism of flowering control by rootstock in scion-stock plants.
Acta Horticulturae Sinica