目的建立柿Diospyros kaki叶的HPLC指纹图谱,并进行多成分测定,为柿叶及其相关产品的质量控制提供科学依据。方法采用HPLC法,Diamonsil C18（250 mm×4.6 mm,5μm）色谱柱;以乙腈-0.2%甲酸水为流动相梯度洗脱;体积流量1.0 m L/min;柱温35℃;检测波长360 nm;进样量10μL;对22批柿叶药材进行指纹图谱研究,用中药色谱指纹图谱相似度评价系统（2004A版）进行评价,系统聚类分析和PCA分析将22批柿叶药材分为两类,同时对金丝桃苷、异槲皮苷、槲皮素和山柰素成分测定方法进行方法学考察。结果建立了柿叶的指纹图谱,共标定了12个色谱峰为柿叶药材指纹图谱的特征峰,金丝桃苷在0.044~0.880μg,异槲皮苷在0.078~1.560μg,槲皮素在0.040~0.800μg,山柰素在0.035~0.700μg线性关系良好,r均大于0.999 8,平均回收率分别为97.59%、98.97%、100.55%、99.96%。结论 HPLC指纹图谱结合多成分测定可为柿叶及其相关产品的质量控制提供参考。
Objective To establish the HPLC fingerprint and to determine multiple components of leaves of Diospyros kaki, in order to provide a scientific basis for quality control for the D. kaki leaves and related products. Methods The chromatographic separate was achieved on a Diamonsil C18（250 mm × 4.6 mm, 5 μm） column with acetonitrile and 0.2% formic acid in water as the mobile phase for gradient elution; Volume flow rate was 1.0 m L/min; Column temperature was 35 oC; Detection wavelength was 360 nm; And injection was 10 μL; On 22 batches of persimmon leaf medicinal materials of fingerprint study, was evaluated with a chromatographic fingerprint similarity evaluation system（version 2004A）, the 22 batches of persimmon leaf are divided into two categories by system cluster analysis and PCA analysis. And the determination method of hyperoside, isoquercitrin, quercetin, and kaempferol was also investigated at the same time. Results The fingerprint of D. kaki leaves was established. There were 12 common peaks in the fingerprint of D. kaki leaves. Hyperoside, isoquercitrin, quercetin and kaempferol were baseline seperated with good linearity relationships between concentration and peak areas over the linear ranges, within 0.044—0.880, 0.078—1.560, 0.040—0.800 and 0.035—0.700 μg（r 〉0.999 8）. The average recoverys of the investigated compounds were 97.59%、98.97%、100.55%、99.96%. Conclusion HPLC fingerprint binding index component determination can provide a reference for the quality of D. kaki leaves and related products control.
Chinese Traditional and Herbal Drugs
leaves of Diospyros kaki Thunb.