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甘蓝型油菜BnMPK3的序列分析及其对环境信号的响应 预览

Sequence Analysis of Brassica napus MAP Kinase 3 and It's Expression Responses to Environmental Signals
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摘要 为了分析油菜MAP激酶3对各种环境胁迫信号的表达响应,以甘蓝型油菜中双9号为试材,采用同源克隆法克隆了甘蓝型油菜MAP激酶3基因(Bn MPK3)的c DNA序列。序列分析表明,Bn MPK3编码一条370个氨基酸残基的蛋白序列,这个序列含有1个高度保守的TEY磷酸化位点和一个CD锚定区域,与At MPK3的序列相似性达到94%。烟草瞬时表达分析显示,Bn MPK3是1个细胞核定位蛋白;荧光定量PCR分析显示,Bn MPK3在茎以及花和叶组织器官中特异性表达,并且对Na Cl溶液、4℃低温、PEG溶液和K2Cr2O7溶液以及甲基茉莉酸(Me JA)和乙烯前体(ACC)等的处理显著上调表达。结果表明,Bn MPK3是高盐、低温、干旱、重金属以及JA和ET等各种环境胁迫因子的响应基因,建议Bn MPK3可能在调控油菜对各种环境胁迫防御的过程中位于JA和ET相关的信号网络中,数据将为进一步研究Bn MPK3在油菜抗逆中的功能及其机制提供理论线索。 In order to explore the effect of environment signals on the expression of MPK3 in oilseed rape,a full length c DNA of Brassica napus MPK3( Bn MPK3) was cloned from the cv. Zhongshuang No. 9 by homology cloning approach. Sequence analysis showed that Bn MPK3 contained a highly conserved motif TEY and a CD domain( common docking domain) and was consisted of 370 amino acid residues exhibiting 94% identity with At MPK3. Transient expression assays in tobacco showed that Bn MPK3 is localized in nuclei. Quantitative RT-PCR analysis showed that Bn MPK3 was especially expressed in stems,flowers and leaves and revealed that its expression was significantly up-regulated by the treatments with Na Cl solution,4 ℃,PEG4000 solution,K2Cr2O7 solution or Me JA,ACC solution. The results demonstrated that Bn MPK3 was a response gene involved in responses to various environmental stresses including high-salinity,low temperature,drought and heavy metal pollution and to signaling molecules JA and ET,suggesting that Bn MPK3 may involve in JA and ET signaling pathways in defense response against various environmental stresses in oilseed rape,which will provide useful information in further in-depth functional analysis of Bn MPK3 in defense to environmental stresses.
作者 陈婷 方和娣 李霄 李冠英 谭小力 张志燕 王政 CHEN Ting, FANG Hedi, LI ( Institute of Life Xiao, LI Guanying, TAN Xiaoli, ZHANG Zhiyan, WANG Zheng Sciences,Jiangsu University,Zhenjiang 212013,China)
出处 《华北农学报》 CSCD 北大核心 2016年第5期94-100,共7页 Acta Agriculturae Boreali-Sinica
基金 国家自然科学基金面上项目(31071672) 江苏大学高级专业人才科研启动基金项目(09JDG061)
关键词 甘蓝型油菜 BnMPK3 表达响应 环境胁迫 Brassica napus BnMPK3 Expression responses Environmental stresses
作者简介 陈婷(1991-),女,江苏苏州人,在读硕士,主要从事油菜功能基因组学研究。 通讯作者:王政(1974-),男,内蒙古包头人,副研究员,博士,主要从事油菜功能基因组学研究。
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参考文献23

  • 1MAPK Group. Mitogen-activated protein kinase cascades in plants:a new nomenclature [ J ]. Trends in Plant Sci- ence, 2002,7 ( 7 ) : 301 - 308. 被引量:1
  • 2赵琳琳,徐启江,姜勇,李玉花.生物和非生物胁迫下的植物细胞中丝裂原活化蛋白激酶(MAPK)信号转导[J].植物生理学通讯,2008,44(1):169-174. 被引量:13
  • 3Jonak C, Okr~sz L, B~gre L, et al. Complexity, cross talk and integration of plant MAP kinase signalling [ J 1. Cur- rent Opinion in Plant Biology,2002,5(5 ):415 -424. 被引量:1
  • 4张腾国,刘玉冰,夏小慧.植物MAP激酶级联途径研究进展[J].西北植物学报,2008,28(8):1704-1714. 被引量:10
  • 5梁湾湾..油菜与拟南芥中MAPK级联途径应答逆境的分子机制研究[D].西北农林科技大学,2014:
  • 6Hamel L P, Nicole M C, Sritubtim S, eta|. Ancient sig- nals:comparative genomics of plant MAPK and MAPKK gene families[ J]. Trends in Plant Science ,2006,11 (4) : 192 - 198. 被引量:1
  • 7Matern S,Peskan-Berghoefer T, Gromes R, et al. Imposed glutathione-mediated redox Switch modulates the tobacco wound-induced protein kinase and salicylic acid-induced protein kinase activation state and impacts on defence a- gainst Pseudomonas syringae [ J ]. Journal of Experimental Botany ,2015,66 ( 7 ) : 1935 - 1950. 被引量:1
  • 8Kiegerl S, Cardinale F, Siligan C, et al. SIMKK, a mito- gen-activated protein kinase (MAPK) kinase,is a specif- ic activator of the salt stress-induced MAPK, SIMK [ J ]. The Plant Cell ,2000,12 ( 11 ) :2247 - 2258. 被引量:1
  • 9Xu J, Xie J, Yan C, et al. A chemical genetic approach demonstrates that MPK3/MPK6 activation and NADPH oxidase-mediated oxidative burst are two independent sig- naling events in plant immunity[ J ]. The Plant Journal:for Cell and Molecular Biology,2014,77 ( 2 ) :222 - 234. 被引量:1
  • 10Meskiene I, Baudouin E, Schweighofer A, et al. Stress-in- duced protein phosphatase 2C is a negative regulator of a mitogen-activated protein kinase[ J]. The Journal of Bio- logical Chemistry, 2003,278 ( 21 ) : 18945 - 18952. 被引量:1

二级参考文献111

  • 1Ahlfors R, Macioszek V, Rudd J, Brosche M, Schlichting R, Scheel D, Kangasjarvi J (2004). Stress hormone-independent activation and nuclear translocation of mitogen-activated protein kinases in Arabidopsis thaliana during ozone exposure. Plant J, 40:512-522 被引量:1
  • 2Apel K, Hirt H (2004). REACTIVE OXYGEN SPECIES: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol, 55:373-399 被引量:1
  • 3Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Boiler T, Ausubel FM, Sheen J (2002). MAP kinase signalling cascade in Arabidopsis innate immunity. Nature, 415:977-983 被引量:1
  • 4Cheong YH, Moon BC, Kim JK, Kim CY, Kim MC, Kim IH, Park CY, Kim JC, Park BO, Koo SC et al. (2003). BWMK1, a rice mitogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor. Plant Physiol, 132:1961-1972 被引量:1
  • 5Desikan R, Hancock JT, Ichimura K, Shinozaki K, Neill SJ (2001). Harpin induces activation of the Arabidopsis mitogen-activated protein kinases AtMPK4 and AtMPK6. Plant Physiol, 126:1579-1587 被引量:1
  • 6Droillard M, Boudsocq M, Barbier-Brygoo H, Lauriere C (2002). Different protein kinase families are activated by osmotic stresses in Arabidopsis thaliana cell suspensions. Involvement of the MAP kinases AtMPK3 and AtMPK6. FEBS Lett, 527:43-50 被引量:1
  • 7Feilner T, Hultschig C, Lee J, Meyer S, Immink RG, Koenig A, Possling A, Seitz H, Beveridge A, Scheel D et al (2005). High throughput identification of potential Arabidopsis mitogenactivated protein kinases substrates. Mol Cell Proteomics, 4:1558-1568 被引量:1
  • 8Hadiarto T, Nanmori T, Matsuoka D, Iwasaki T, Sato K, Fukami Y, Azuma T, Yasuda T (2006). Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1-AtMEK1 pathway by wounding. Planta, 223: 708-713 被引量:1
  • 9He C, Fong SH, Yang D, Wang GL (1999). BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice. Mol Plant Microbe Interact, 12:1064-1073 被引量:1
  • 10Ichimura K, Casais C, Peck SC, Shinozaki K, Shirasu K (2006). MEKK1 is required for MPK4 activation and regulates tissue-specific and temperature-dependent cell death in Arabidopsis. J Biol Chem, 281:36969-36976 被引量:1

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