期刊文献+

降胆固醇乳酸杆菌的筛选及对小鼠血清胆固醇的影响 预览 被引量:3

Screening of Lactobacillus with Cholesterol-Degrading Property and Its Effect on Serum Cholesterol in Mice
在线阅读 下载PDF
收藏 分享 导出
摘要 目的:从羊新鲜粪便中分离筛选具有降胆固醇能力的乳酸杆菌,探讨其对小鼠血清胆固醇含量的影响。方法:以碳酸钙-MRS培养基和胆固醇-MRS培养基筛选具有高效降胆固醇能力的乳酸杆菌;利用形态特征、生理生化特性和16SrDNA序列分析鉴定分离菌株L-3。为了验证其效果,将40只8周龄雄性BALB/c小鼠随机分为基础饲料组(A)、高脂模型组(B)、高脂模型+生理盐水组(C)、高脂模型+菌株L-3组(D)。分别饲喂第15、30天,采血,测定总胆固醇(totalcholesterol,TC)、总甘油三酯(triglyceride,TG)和高密度脂蛋白胆固醇(highdensitylipoprotein-cholesterol,HDL-C)的含量。结果:筛选得到一株能够高效降解胆固醇的菌株L-3,胆固醇降解率达到(35.93±0.43)%,经鉴定为植物乳杆菌。饲喂第15天,成功构建出高脂血症小鼠模型。灌胃第30天,与B组相比,D组TC、TG、动脉硬化指数水平均极显著降低(P〈0.01),HDL-C水平显著升高(P〈0.05),HDL-C/TC水平极显著升高(P〈0.01),而对照组几乎无降胆固醇效果(P〉0.05)。结论:实验获得了1株在体内外均具有高效降胆固醇能力的植物乳杆菌LactobacillusplantarumL-3,为进一步开发其为功能性微生态制剂提供了可能。 Objective: To screen Lactobacillus with high cholesterol-degrading capacity from fresh sheep feces andexplore the effect of the screened strain on serum cholesterol level in hypercholesterolemic mice. Methods: We screenedLactobacillus with high cholesterol-reducing capacity using Calcium Carbonate-Man-Rogosa-Sharp (MRS) mediumand cholesterol-MRS medium. The screened strain, named as L-3, was identified by colony morphology, physiologicaland biochemical tests and 16S rDNA sequencing. A feeding experiment was conducted on forty Kunming male mice(eight weeks old). The mice were randomly divided 4 groups: A, B, C and D, which were fed with normal diet, highfat diet (HFD), HFD plus normal saline and HFD plus strain L-3, respectively. The serum levels of total cholesterol(TC), triglyceride (TG), and high density lipoprotein-cholesterol (HDL-C) were determined on the 15th and 30th day ofthe experiment. Results: This selected strain was identified as Lactobacillus plantarum L-3 and the degradation rateof cholesterol by the stain could reach up to (35.93 ± 0.43)%. A hypercholesterolemic mice model was successfullyconstructed after feeding a high-cholesterol diet for 15 days. Compared with group B, serum TC, TG and AI levelswere extremely significantly decreased in group D after 30 days of oral administration of strain L-3 (P 〈 0.01), HDL-Clevels of group D was significantly increased (P 〈 0.05), and HDL-C/TC levels were extremely significantly increased(P 〈 0.01). However, the negative control group did not show cholesterol degradation (P 〉 0.05). Conclusion: L. plantarumL-3 has probiotic potential as indicated by its hypocholesterolemic effect both in vivo and in vitro.
作者 丁盼盼 丁轲 余祖华 李旺 李元晓 刘一尘 何万领 赵战勤 王玉琴 程相朝 张春杰 DING Panpan, DING Ke, YU Zuhua, LI Wang, LI Yuanxiao, LIU Yichen, HE Wanling, ZHAO Zhanqin, WANG Yuqin,CHENG Xiangchao, ZHANG Chunjie(1. Hongxiang Biological Feed Laboratory, Henan University of Science and Technology, Luoyang 471003, China;2. Key Laboratory of Animal Disease and Public Health of Henan Province, Luoyang 471003, China;3. Research Center of Breeding Engineering Technology for Meat Sheep of Henan Province, Luoyang 471003, China)
出处 《食品科学》 EI CAS CSCD 北大核心 2016年第15期192-197,共6页 Food Science
基金 河南省科技厅科技攻关项目(162102110064) 河南省教育厅科学技术研究重点项目(14B230002)
关键词 乳酸杆菌 降胆固醇 小鼠 筛选 Lactobacillus cholesterol degradation mice screening
作者简介 丁盼盼(1987-),女,硕士研究生,主要从事动物微生态学研究.E-mail:dpl1021@163.com 通信作者:丁轲(1977-),男,副教授,博士,主要从事动物微生态与动物传染病学研究.E-mail:keding19@163.com
  • 相关文献

参考文献6

二级参考文献75

共引文献58

同被引文献21

引证文献3

二级引证文献3

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈