目的探讨碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）对多孔钽颗粒修复犬颌骨缺损过程中的促进效果。方法 Beagle犬6只,建立下颌骨缺损模型共计36个,单侧3个骨缺损分别为多孔钽颗粒修复组（实验组A）、bFGF＋多孔钽颗粒修复组（实验组B）、无干预组（对照组）。术后4、8、12周各处死2只犬,行大体、X线、组织学观察及新生骨面积统计分析。结果 X线片示实验组与周围骨质结合良好,对照组12周后仍可见低密度骨缺损区域。甲苯胺蓝染色示术后8周及12周实验组B骨连续性及成熟度优于实验组A。术后4周实验组B新生骨形成面积明显大于实验组A（P〈0.05）,而8周末实验组A明显大于实验组B（P〈0.05）。结论 b FGF可增强多孔钽颗粒的骨修复能力。
Objective To evaluate the effect of basic fibroblast growth factor（ b FGF） on the repair of canine jaw defects by porous tantalum particles. Methods A total of 36 mandibular defect models were established in 6 Beagle dogs,and the unilateral bone defects were implanted with porous tantalum（ group A）and porous tantalum loaded with b FGF（ group B）. The control group did not perform intervention.Mandibular specimens were harvested in 4,8 and 12 weeks postoperatively to carry out gross observation,X-ray analysis,fluorescence microscopy and histological examinations,and the ossification in the defects at different time was observed. Results After 12 weeks,the porous tantalum particles were surrounded by new bone in the experimental groups,and the defect area was still visible in the control group. The continuity of the bone around the porous tantalum particles in group B was superior than that in group A at the end of 12 weeks. New bone formation area in group B was greater than that in group A in 4 weeks after operation（ P 〈0. 05）,while group A was greater than group B at the end of 8 weeks（ P 〈0. 05）. Conclusion When repairing the bone defects with porous tantalum particles,b FGF can promote the osteogensis,and shorten the bone healing time.
Acta Academiae Medicinae Militaris Tertiae