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红景天苷对帕金森病轻度认知功能损害改善作用的研究

Effects of salidroside on mild cognitive impairment of Parkinson’s disease
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摘要 目的:明确红景天苷对帕金森病认知功能障碍(PD-MCI)的改善作用,并对作用机制做初步探讨,为治疗PD-MCI新药研发提供依据。方法:选取C57BL/6小鼠60只,分为对照组、MPTP组及红景天苷组。通过震颤评分和爬杆实验检测小鼠的行为学变化;应用水迷宫实验观察红景天苷对认知功能的改善状况;应用乙酰胆碱酯酶(AchE)活性试剂盒测脑组织乙酰胆碱酯酶活性;应用Tunel试剂盒检测脑内黑质、纹状体的神经元凋亡情况。结果:与MPTP组比较,第3天到第6天,红景天苷组逃避潜伏期明显缩短(P<0.05)。在多巴胺(DA)及Ach含量测定中,红景天苷组DA含量相对于MPTP组显著提升(P<0.05),AchE活性较MPTP组显著下降(P<0.05)。显著延缓小鼠脑组织黑质的凋亡程度(P<0.05)。结论:红景天苷对PD轻度认知功能损害有保护作用。 Objective:To clarify the improvement effect of salidroside towards Parkinson’s disease mild cognitive impairment(PD-MCI)and discuss its working mechanisms and provide evidence for the drug discovery on PD-MIC.Methods:Sixty C57 BL/6 mice were divided into 3 groups:MPTP group,blank control group and salidroside group.The behavioral changes of mice were detected by tremor score and pole-climbing test.The effects of salidroside on PD-MCI were observed through water maze test.The activity of acetylcholinesterase in mouse brain tissue was tested by acetylcholinesterase activity kit.The neuronal apoptosis in the substantia nigra and striatum was detected by Tunel kit.Results:In the water maze test,from the 3 rd to the 6 th day,the salidroside group significantly shortened the escape latency than MPTP group(P<0.05).In the test experiment of dopamine and acetylcholine,compared with the MPTP group,dopamine level was increased in salidroside group(P<0.05);acetylcholine activity in the salidroside group was significantly decreased(P<0.05).In the apoptosis experiment,compared with the MPTP group,salidroside could significantly reduce the degree of apoptosis in substantia nigra tissue of mice(P<0.05).Conclusion:Salidroside has a protective effect on mild cognitive impairment in Parkinson’s disease.
作者 陈钧 韩明伟 付屺瞻 韩祖成 CHEN Jun;HAN Ming-wei;FU Qi-zhan;HAN Zu-cheng(Department of Encephalopathy,Shaanxi Traditional Chinese Medicine Hospital,Xi’an710003,China;No.1CadetBrigade,Fourth Military Medical University,Xi’an710032,China;Graduate College,Shaanxi University of Chinese Medicine,Xianyang712046,China)
出处 《中华中医药杂志》 CAS CSCD 北大核心 2019年第6期2455-2459,共5页 China Journal of Traditional Chinese Medicine and Pharmacy
基金 陕西省创新人才计划项目(No.2017KJXX-84) 陕西省自然科学基金面上项目(No.2016JM8014) 陕西省中医药管理局课题(No.15-LC019).
关键词 红景天苷 帕金森病 MPTP 认知功能损害 Salidroside Parkinson’s disease MPTP Mild cognitive impairment
作者简介 通讯作者:陈钧,陕西省西安市西华门4号陕西省中医医院脑病科,邮编:710003,电话:029-87251941,E-mail:chj2002819@163.com
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