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A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells
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摘要 In this study, a resorufin derivative RTP-1, which is a novel fluorescent ‘‘turn-on’’ probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester group of the probe by hydrazine led to a prominent enhancement of fluorescent intensity, as well as a remarkable color change from colorless to pink, which could be distinguished by naked eye. The fluorescence enhancement showed decent linear relationship with hydrazine concentration ranging from 0 to 50 mmol/L, with a detection limit of 0.84 mmol/L. The specificity of RTP-1 for hydrazine to a number of metal ions, anions and amines is satisfactory. The sensing mechanism of RTP-1 and hydrazine was evaluated by HPLC, ESI mass spectrometry and density functional theory(DFT).Moreover, we have utilized this fluorescent probe for imaging hydrazine in living cells, and the fluorescence was clearly observed when the cells were incubated with hydrazine(100 mmol/L) for 30 min. In this study, a resorufin derivative RTP-1, which is a novel fluorescent ‘‘turn-on'' probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester group of the probe by hydrazine led to a prominent enhancement of fluorescent intensity, as well as a remarkable color change from colorless to pink, which could be distinguished by naked eye. The fluorescence enhancement showed decent linear relationship with hydrazine concentration ranging from 0 to 50 mmol/L, with a detection limit of 0.84 mmol/L. The specificity of RTP-1 for hydrazine to a number of metal ions, anions and amines is satisfactory. The sensing mechanism of RTP-1 and hydrazine was evaluated by HPLC, ESI mass spectrometry and density functional theory(DFT).Moreover, we have utilized this fluorescent probe for imaging hydrazine in living cells, and the fluorescence was clearly observed when the cells were incubated with hydrazine(100 mmol/L) for 30 min.
作者 Tun Tang Yu-Qi Chen Bo-Shi Fu Zhi-Yong He Heng Xiao Fan Wu Jia-Qi Wang Shao-Ru Wang Xiang Zhou Tun Tang;Yu-Qi Chen;Bo-Shi Fu;Zhi-Yong He;Heng Xiao;Fan Wu;Jia-Qi Wang;Shao-Ru Wang;Xiang Zhou;College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University;
出处 《中国化学快报:英文版》 SCIE CAS CSCD 2016年第4期540-544,共5页 Chinese Chemical Letters
基金 supported by the National Basic Research Program of China (973 Program, Nos. 2012CB720600, 2012CB720603) the National Science Foundation of China (Nos. 91413109, 21202126) East Lake High-tech Zone 3551 Talents Scheme
关键词 选择性检测 活细胞成像 荧光探针 水合肼 CYP2E1 应用 密度泛函理论 HPLC法 Resorufin Fluorescent probe Hydrazine Selectivity Cell imaging
作者简介 Corresponding author. E-mail address: xzhou@whu.edu.cn (X. Zhou).
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参考文献28

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