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弥漫性轴索损伤对大鼠脑皮质钙释放激活钙通道调节分子1表达的影响 预览

Influence of diffuse axonal injury on expression of ORAI1 in brain cortex of rat
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摘要 目的 研究弥漫性轴索损伤(DAI)对大鼠脑皮质钙释放激活钙通道调节分子1(ORAI1)表达的影响。方法利用瞬间旋转损伤装置,建立大鼠DAI模型,分为DAI组及对照组,DAI组又分为DAI组伤后6 h、1 d、3 d、7 d、14 d等5个亚组。运用苏木精-伊红(HE)染色观察大鼠DAI后脑皮质的病理生理变化;运用免疫组化检测大鼠脑皮质ORAI1的分布及表达。结果 HE染色显示,DAI伤后6 h-1 d神经元变性、坏死、崩解数量增多,轴索的断裂和紊乱明显;DAI伤后3-14 d,开始恢复期改变,神经元的变性、水肿和坏死现象逐渐减少。免疫组化检测显示,与对照组比较,DAI伤后6 h大鼠脑皮质ORAI1的表达即开始增加,伤后1 d达高峰,伤后3-14 d逐渐降低,至伤后14 d时仍高于对照组(P〈0.05)。结论 DAI后大鼠脑皮质ORAI1的表达水平呈先增加后降低的趋势,这一变化与大鼠DAI后脑皮质的病理生理变化有关,ORAI1可能参与DAI后的病理生理过程。 Objective To investigate influence of diffuse axonal injury (DAI) on expression of calcium release-activated calcium channel modulator 1 (ORAI1) in brain cortex of rat. Methods Lateral head-rotating device was used to establish DAI model. Rats were randomly divided into control group and DAI groups, which contained five subgroups (6 h, 1 d, 3 d, 7 d, 14 d after injury). HE staining was used to observe pathological change in cerebral cortex after DAI and immunohistochemistry staining was applied to determine the distribution and expression of ORAI1. Results HE staining showed increased neuronal degeneration and necrosis at 6 h-1 d after DAI. Structural distortion of axons were observed, During 3 d to 14 d, neuronal recovery began. Degeneration, edema and necrosis of neuron gradually alleviated. Immunohistochemistry showed increase of ORAI1 expression began at 6 h in cortex after DAI; peaked at 1 d and gradually deceased from 3 d to 1d d; at 14 d, expression of ORAI1 still remained higher than control group (P 〈 0.05). Conclusion ORAI1 expression significantly increase and then decrease in cerebral cortex of rats after DAI. Dynamic change of ORAI1 after DAI positively correlates with the pathological evolution, indicating the possible role of ORAI1 in pathophysiology of DAI.
作者 金涛 王松林 李东波 杨涛 宋锦宁 JIN Tao, WANG Songlin, LI Dongbo, YANG Tao, SONG Jinning( 1.Department of Neurosurgery, Center Hospital of Ankang City, Shaanxi Province, Ankang 725000, China; 2.Department of Neurosurgery, the First AffiliatedHospital of Xi'an Jiaotong University, Shaanxi Province, Xi'an 710061, China)
出处 《中国医药导报》 CAS 2016年第6期22-25,共4页 China Medical Herald
关键词 钙释放激活钙通道调节分子1 大鼠 弥漫性轴索损伤 病理生理 Calcium release-activated calcium channel modulator 1 Rat Diffuse axonal injury Pathophysiology
作者简介 金涛(1980.8-),男,硕士;研究方向:脑外伤的基础与临床。 [通讯作者]宋锦宁(1962.7-),男,博士,教授,主任医师,博士生导师;研究方向;脑血管病及脑外伤的基础与临床。
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