The winter wheat variety ＇Dongnongdongmai 1＇ （＇dnl＇）, cultivated by Northeast Agricultural Uni- versity with independent intellectual property rights, was strong cold tolerance （resistance -30 ℃ low tempera- ture） cultivars. This study analysed and forecasted miRNA and its target genes related to cold resistance from ＇dnl＇ EST by BLAST, UNAFOLD bioinformatics software. Quantitative RT-PCR were applicated to vertify ex- pression characteristic of miRNAs and their target genes which had high expression abundance and apparent differentiation. The result showed that 16 miRNAs, belonging to 15 families and corresponding 137 target genes, could respond to cold stress. Among them miR1118, miR1120, miR1128, miR1133 and miR1139 had higher expression abundance and more apparent differentiation, whose expression levels were significantly de- creased at -10 ℃ and significantly rose at -25 ℃. The expression level of their corresponding target genes were significantly increased at -10 ℃, while decreased significantly at -25 ℃. It was suggested that the selected five miRNA and their target genes could respond to low temperature stress, which associated with the strong cold tolerance of ＇dnl ＇. This study laid the foundation for molecular mechanism of microRNAs regulation in winter wheat under low temperature stress response.
Plant Physiology Communications