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Glil基因对人牙周膜干细胞增殖及成骨分化调控机制的研究 预览 被引量:2

Regulatory mechanism of Glil gene on human periodontal ligament stem cells on proliferation and osteogenic differentiation
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摘要 目的:通过构建Glil基因腺病毒载体,以此上调人牙周膜干细胞( PDLSCs)中Glil基因的表达,从而探讨其对人PDLSCs增殖及成骨分化的影响。方法将Glil目的基因亚克隆至腺病毒载体,并将含目的基因的病毒转染PDLSCs细胞。 CCK-8法检测过表达Glil对PDLSCs增殖的影响,利用Western blot检测PDLSCs中Glil的表达水平及其成骨相关标志物ALP、Runx2的表达。结果成功构建了过表达Glil目的基因的腺病毒载体并转染PDLSCs,过表达Glil的PDLSCs与空载体组比较,增殖速率减慢(P=0.003),Western blot检测结果显示PDLSCs过表达Glil目的基因后可以高表达成骨分化标记蛋白ALP、Runx2,差异具有统计学意义(P〈0.05)。结论过表达Glil基因会导致PDLSCs的增殖减慢,成骨相关标志物增加,说明Glil基因能使PDLSCs成骨分化能力增强。 Objective To up-regulate the expression of Glil gene in periodontal ligament stem cells ( PDLSCs) and to explore the effect of Glil gene on PDLSCs proliferation and osteogenesis differentiation by establishing Glil gene adenovirus vectors. Methods Subcloned Glil to viral backbone vector Adtrack-CMV and transfered the established vector to 293T cells, which was to acquire the virus particles. Trans-fected aim cells,namely PDLSCs,with these virus. Detected its effect on PDLSCs proliferation with CCK-8 assay, and detected the expression of Glil and the bone-related markers ALP and Runx2 through Western blot. Results An adenovirus vector, which were over expressed Glil gene, was successfully constructed and transfected to PDLSCs. Compared with the empty vector group and normal group, the over expressed one had a much slower proliferation rate in CCK-8 assay (P=0. 003). Western blot showed that ALP and Runx2 can be overexpressing os-teogenic differentiated after PDLSCs successfully transfected with the Glil gene. Conclusion Over expressing Glil gene would lead to a much slower proliferation rate in the PDLSCs and an increase of the bone-related markers. It is concluded that Glil can enhance the osteogenic dif-ferentiation capacity in PDLSCs.
作者 罗金英 钟建鑫 舒绍兵 张洁 朱璨 张倩 汤玲 周继祥 LUO Jin-ying,ZHONG Jian-xin,SHU Shao-bing,ZHANG Jie,ZHU Can,ZHANG qian,TANG Ling,ZHOU Ji-xiang(1. Department of Stomatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China ; 2. Department of Stomatology, Nanchuan People' s Hospital, Chongqing 408415, China ;3. Department of Stomatology, Authority Clinic of Chongqing Armed Police Force, Chongqing 401147, China ;4. College of Biomedical Engineering, Chongqing University, Chongqing 400044, China)
出处 《局解手术学杂志》 2015年第3期245-248,共4页 Journal of Regional Anatomy and Operative Surgery
基金 国家自然科学基金面上项目(30870599)
关键词 人牙周膜干细胞 Glil 增殖 成骨分化 human periodontal ligament stem cells Glil proliferation osteogenic differentiation
作者简介 [通讯作者]周继祥,E—mail:Zboujx6518@sina.com
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参考文献17

  • 1Choi JK,Hwang HI,Jang YJ.The efficiency of the in vitro osteo/dentinogenic differentiation of human dental pulp cells,periodontal ligament cells and gingival fibroblasts[J].Int J Mol Med,2015,35(1):161-168. 被引量:1
  • 2Zhang C,Li J,Zhang L,et al.Effects of mechanical vibration on proliferation and osteogenic differentiation of human periodontal ligament stem cells[J].Arch Oral Biol,2012,57(10):1395-1407. 被引量:1
  • 3Kim SY,Yoo JY,Ohe JY,et al.Differential expression of osteo-modulatory molecules in periodontal ligament stem cells in response to modified titanium surfaces[J].Biomed Res Int,2014,(2014):452175.doi:10.1155/2014/452175. 被引量:1
  • 4常慧君,申涛,董世武,杨彦春,张洁,周继祥.purmorphamine促进人牙周膜干细胞应力成骨[J].第三军医大学学报,2012,34(9):839-842. 被引量:5
  • 5张洁,常慧君,李雨轩,舒绍兵,罗金英,徐志玲,汤玲,杨彦春,周继祥.Smoothened慢病毒干扰载体的构建及其对人牙周膜干细胞增殖与分化的影响[J].第三军医大学学报,2013,35(11):1133-1136. 被引量:2
  • 6Liu Y,Wang H,Cheng G,et al.Experssion analysis of Gli1 and Gli2 in different tissues and muscle-derived cells of Qinchuan cattle[J].Genet Mol Res,2014,13(4):8767-8775. 被引量:1
  • 7Kitaura Y,Hojo H,Komiyama Y,et al.Glil haploinsufficency leads to dereased bone mass with an uncoupling of bone metabolism in adult mice[J].Plo S One,2014,9(10):e109597. 被引量:1
  • 8Deng C,Yu Y.Roles of Hh signaling pathway on injury of bone with fluorosis[J].Zhonghua Bing Li Xue Za Zhi,2014,43(1):68-70. 被引量:1
  • 9Choudhry Z,Rikani AA,Choudhry AM,et al.Soinc Hh signaling pathway:a complex network[J].Ann Neurosci,2014,21(1):28-31. 被引量:1
  • 10Ohata Y,Ozono K.Bone and stem cells.The mechanism of osteogenic differentiation from mesenchymal stem cell[J].Clin Calcium,2014,24(4):501-508. 被引量:1

二级参考文献59

  • 1彭庭莉,周继祥,杨军,杨彦春,张从纪.动态张应力对人牙周膜成纤维细胞MMP-9 mRNA合成的影响[J].中华口腔医学杂志,2005,40(6):462-462. 被引量:2
  • 2Katagiri T, Yamaguchi A, Ikeda T, et al. The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bonemorphogenetic protein-2. Biochem Biophy Res Commun, 1990, 172 ( 1): 295-299. 被引量:1
  • 3Hu H, Hilton MJ, Tu X, et al. Sequential roles of Hedgehog and Wnt signaling in osteoblast development. Development, 2005, 132(1): 49-60. 被引量:1
  • 4Lee JJ, von Kessler DP, Parks S, et al. Secretion and localized transcription suggest a role in positional signaling for products of the segmentation gene hedgehog. Cell, 1992, 71 (1): 33-50. 被引量:1
  • 5Marigo V, Davey RA, Zuo Y, et al. Biochemical evidence that patched is the Hedgehog receptor. Nature, 1996, 384(6605): 176-179. 被引量:1
  • 6Murone M, Rosenthal A, de Sauvage FJ. Sonic hedgehog signaling by the patched-smoothened receptor complex. Curt Biol, 1999, 9(2): 76-84. 被引量:1
  • 7Ingham PW. Transducing Hedgehog: the story so far. EMBO J, 1998, 17(13): 3505-3511. 被引量:1
  • 8Bitgood MJ, McMahon AP. Hedgehog and Bmp genes are coexpressed at many diverse sites of cell-cell interaction in the mouse embryo. Dev Biol, 1995, 172(1): 126-138. 被引量:1
  • 9McMahon AP, Ingham PW, Tabin CJ, et al. Developmental roles and clinical significance of hedgehog signaling. Curr Top Dev Biol, 2003, 53: 1-114. 被引量:1
  • 10Chiang C, Litingtung Y, Lee E, et al. Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function. Nature, 1996, 383(6599): 407-413. 被引量:1

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