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子宫内膜异位症患者病变部位ZEB1和ZEB2表达水平

Expression and clinical significance of ZEB1 and ZEB2 in lesions of endometriosis
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摘要 目的探讨E-盒结合锌指蛋白1(ZEB1)、ZEB2在子宫内膜异位症患者中的表达水平。方法收集2017年10月至2018年3月于本院就诊的46例子宫内膜异位症患者作为观察组,另选取30例因卵巢良性肿瘤行手术治疗且病理证实非子宫内膜异位症患者作为对照组,采用RT-PCR法、Western-blot法测定子宫内膜中ZEB1、ZEB2的相对表达量,进一步分析ZEB1、ZEB2表达水平与子宫内膜异位症美国生殖医学学会修订本评分(r ASRM)分期的关系。结果 RT-PCR法测得观察组ZEB1、ZEB2的mRNA相对表达量分别为(0.675±0.070)、(0.620±0.080),对照组ZEB1、ZEB2的m RNA相对表达量分别为(0.065±0.007)、(0.080±0.008),观察组ZEB1、ZEB2 m RNA表达水平明显高于对照组,差异有统计学意义(P<0.05);Western-blot法测得观察组ZEB1、ZEB2的蛋白相对表达量分别为(16.40±1.30)、(18.04±1.52),对照组中ZEB1、ZEB2的蛋白相对表达量分别为(1.50±0.70)、(1.79±0.75),观察组ZEB1、ZEB2蛋白表达水平明显高于对照组,差异有统计学意义(P<0.05)。ZEB1、ZEB2表达水平与内异症rASRM分值无明显关系(P>0.05)。结论 ZEB1、ZEB2在子宫内膜异位症患者中高表达,可考虑作为子宫内膜异位症患者治疗效果的指标。 Objective To explore prognostic value of zinc finger E-box-binding protein 1(ZEB1),ZEB2 expression in endometriosis(EMs). Methods 46 tissue specimens of EMs were collected as the observing group, and 30 normal tissue specimens of normal endometrium were collected as the control group. ZEB1/ZEB2 expression levels in tissue were detected by RT-PCR method and Western blot method. The relationship between the expression of ZEB1/ZEB2 and the Revised American Society for Reproductive Medicine(rASRM) was analyzed. Results ZEB1 mRNA and ZEB2 mRNA expression levels measured by RT-PCR in the observing group were(0.675 ±0.070) and(0.620±0.080),respectively;in the control group were(0.065 ±0.007) and(0.080 ±0.008),respectively. ZEB1 mRNA and ZEB2 mRNA expression levels in the observing group were significantly higher than those in the control group(P<0.05). ZEB1 protein and ZEB2 protein expression levels measured by western blot in the observing group were( 16.40± 1.30) and( 18.04± 1.52),respectively;in the control group were(1.50 ±0.70) and( 1.79 ± 0.75),respectively. ZEB1 protein and ZEB2 protein expression levels measured by western blot in the observing group were significantly higher than those in the control group(P<0.05), but there was no obvious relationship between ZEB1, ZEB2 and rASRM of the EMs. Conclusion ZEB1 and ZEB2 were highly expressed in EMs tissues, and could be used as an independent prognostic indicator for patients with EMs.
作者 张春云 张永鹏 ZHANG Chun-yun;ZHANG Yong-peng(Department of Obstetrics and Gynecology,Dongying People's Hospital,Dongying,Shandong 276500;Emergency Department,Dongying People's Hospital,Dongying,Shandong 276500,China)
出处 《热带医学杂志》 CAS 2019年第1期43-45,54共4页 Journal Of Tropical Medicine
关键词 子宫内膜异位症 ZEB1 ZEB2 Endometriosis ZEB1 ZEB2
作者简介 张春云(1978-),女,研究生,主治医师,从事子宫内膜异位症的诊治,E-mail:rmyyzyp2009@163.com.
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