目的：探讨姜黄素对胶质瘤细胞系U87细胞增殖及迁移能力的影响。方法应用CCK-8比色法检测经正常培养液及含不同浓度（25、50、100μmol/L）姜黄素的培养液培养24、48、72 h后的U87细胞的存活率；应用细胞划痕实验检测100μmol/L姜黄素对U87细胞迁移能力的影响；将20只SD大鼠制备成胶质瘤模型,随机分成对照组及实验1、2、3组,每组5只,模型制备后第7天对照组给予生理盐水灌胃,实验1、2、3组分别给予姜黄素100、200、300 mg/kg灌胃,1/d,连续14 d,第15天用microPET-CT对载瘤大鼠肿瘤体积进行测定。结果 U87细胞存活率随着培养液中姜黄素浓度的升高和培养时间的延长而降低（ P〈0．05）；给予100μmol/L姜黄素的培养液培养24和48 h后U87细胞迁移度均低于空白对照组（P〈0．05）；实验2、3组肿瘤体积均小于实验1组和对照组（P〈0．05）。结论姜黄素可抑制胶质瘤U87细胞的增殖及迁移。
Objective To explore the effects of Curcumin on cell proliferation and migration ability of glioma U87 cells. Methods Survival rates of glioma U87 cells, cultured by normal culture solutions and different concentra-tions （25, 50 and 100μmol/L） Curcumin solution were detected using CCK-8 assay after culturing for 24 h, 48 h and 72 h;effect of cell migration by 100 μmol/L Curcumin solution was assessed using cell scuffing test; 20 Sprague-Dawley （SD） rats were randomly divided into control group （n=5） and three experiment groups （group A, group B, group C, each n= 5） after establishing glioma models. The 7th d after establishing models, control group was lavaged with saline solution, and group A, B and C was lavaged with 100, 200 and 300 mg/kg Curcumin solution respectively, （1 time/ d） for 14 d. Tumor volumes were measured on the 15th d using microPET-CT method. Results Survival rates of glioma U87 cells were decreased with elevated Curcumin concentration and lengthened cultivation time （P〈0. 05）;level of U87 cell migration was lower than that in control group after being cultured with Curcumin solution （100μmol/L） for 24 h and 48 h （P〈0. 05）;tumor volumes in group B and C were smaller than those in group A and control group （P〈0. 05）. Con-clusion Curcumin may inhibit the cell proliferation and migration in glioma U87 cells.
Medical Journal of Beijing Military Region