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儿童呼吸道流感嗜血杆菌分离株耐药性与β-内酰胺酶基因研究 被引量:8

Antimicrobial resistance and beta-lactamase gene of Haemophilus influenzae isolates from respiratory tracts in children
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摘要 目的调查儿童呼吸道流感嗜血杆菌分离株的耐药性和产β-内酰胺酶情况,研究分离株β-内酰胺酶基因的特征。方法 2011年1月到2012年6月,从儿童呼吸道分离流感嗜血杆菌,用微量肉汤稀释法测定常用抗生素最低抑菌浓度;用Nitrocefin纸片法检测β-内酰胺酶;用PCR扩增结合限制性内切酶酶切分析对分离株β-内酰胺酶基因进行分型;比较TEM+菌株和ROB-1+菌株、TEM-1+菌株和TEM-2+菌株对常用抗生素的耐药性。结果 537株流感嗜血杆菌的β-内酰胺酶产酶率为52.3%(281/537);产酶株对氨苄西林、头孢克洛、头孢呋辛的MIC50、MIC90和耐药率明显高于非产酶株(P〈0.05);产酶株TEM基因阳性率为91.8%(258/281),其中90.3%为TEM-1型(233/258),7.4%为TEM-2型(19/258),ROB-1基因阳性率为8.2%(23/281);ROB-1+菌株对氨苄西林和阿莫西林/克拉维酸的MIC50和MIC90高于TEM+菌株,但头孢菌素类的MIC50和MIC90低于TEM+菌株;除头孢呋辛外,TEM-1+菌株和TEM-2+菌株对β-内酰胺类的MIC50和MIC90差异无统计学意义。结论成都地区儿童呼吸道流感嗜血杆菌分离株β-内酰胺酶产酶率较高,产酶株主要携带TEM-1基因,对氨苄西林、头孢克洛和头孢呋辛等β-内酰胺类的影响明显。 Objective To investigate antimicrobial resistance and beta-lactamase production of Haemophilus influenzae isolates from respiratory tracts in children and the characteristics of beta-lactamase gene.Methods From January 2011 to June 2012,Haemophilus influenzae were isolated from respiratory tracts in children.Minimum inhibitory concentrations(MICs) of commonly-used antibiotics were determined by micro broth dilution assay,and beta-lactamase production was detected by Nitrocefin disk test.PCR combining restriction endonuclease analysis was employed to do the beta-lactamase genotyping.Antimicrobial resistance was compared between TEM+ isolates and ROB-1+ isolates as well as between TEM-1+ isolates and TEM-2+ isolates.Results 52.3% of the isolates were beta-lactamase positive(281/537),the MIC values(MIC50/MIC90) and resistant rates(R%) of which were higher than those of non beta-lactamase producing isolates against ampicillin,cefaclor and cefuroxime.91.8% of the isolates were TEM gene positive(258/281),consisting of 93.0% of TEM-1+ isolates(233/258),7.4% of TEM-2+ isolates(19/258),and 8.2% of ROB-1+ isolates(23/281).The MIC50 and MIC90 values of ampicillin and amoxicillin/clavulanate against ROB-1+ isolates were higher than those against TEM+ isolates,while those of cephalosporins against ROB-1+ isolates were lower than those against TEM+ isolates.No difference was observed in MIC values between TEM-1+ isolates and TEM-2+ isolates except cefuroxime.Conclusion There is high prevalence of beta-lactamase producing Haemophilus influenzae isolates from respiratory tracts in Chengdu children;the dominant genotype of beta-lactamase producing isolates is TEM-1,which has obvious influences on some beta lactams such as ampicillin,cefaclor and cefuroxime.
作者 谢成彬 王频佳 吴雨露 颜源 易娟 苏喆 XIE Cheng-bin,WANG Pin-jia,WU Yu-lu,YAN Yuan,YI Juan,SU Zhe(1.Sichuan Institute of Maternal and Child Health,Chengdu 610031,China;2.Chengdu Medical College,Chengdu 610083,China)
出处 《中国微生态学杂志》 CAS CSCD 2013年第5期561-565,共5页 Chinese Journal of Microecology
基金 四川省卫生厅科研课题(120493)
关键词 流感嗜血杆菌 耐药性 Β-内酰胺酶 基因 Haemophilus influenzae Antimicrobial resistance beta-lactamase Gene
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