Objective： To investigate the molecular mechanisms of impaired skin barrier function in an atopic der- matitis like model of BALB/c mouse induced by 2, 4- dinitrochloro- benzene （DNCB）, through theanalysis of gene expression related to epidermal differentiation and degeneration. Methods： Mice in dermatitis group were sensitized twice and challenged twice with DNCB. Data were obtained 24 hours after the second challenge. Results： Mice in dermatitis group has significandy higher TEWL and lower stratum comeum hydration than control group. The protein expression of FIG, IVL and TGM1 in epidermis was decreased in dermatitis group, and the protein expression of KLK7 in the outermost layer of epidermis was increased in dermatitis group. The mRNA expression of FLG, IVL, TGM1 and KLK7 resembled the changes of repair phase after tissue damage. Conclusion： Skin barrier function of atopic dermatitis like model in BALB/c mouse induced by DNCB was impaired, and there is an alteration of the ex- pression of gene related to epidermal differentiation and degeneration.
China Journal of Leprosy and Skin Diseases
gene related to epidermal differentiation and degener-ation