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HPLC同时测定败酱草中常春藤皂苷元、齐墩果酸和熊果酸的含量 被引量:20

Simaltanous Determination of Hederagenina,Oleanolic Acid and Ursolic Acid in Herba Patriniae by HPLC
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摘要 目的:建立高效液相色谱法同步测定败酱草败酱药材中常春藤皂苷元、齐墩果酸和熊果酸的含量。方法:采用反相高效液相色谱法,色谱柱为Ultimate C18(4.6 mm×250 mm,5μm);以甲醇-0.2%乙酸铵水溶液为流动相,进行梯度洗脱,检测波长为210 nm,流速为1.0 mL·min-1,柱温为25℃。结果:常春藤皂苷元、齐墩果酸和熊果酸在0.026~0.26,0.082~0.82,0.079~0.79 g·L-1具有良好线性关系(r〉0.999 8),平均回收率分别为100.62%(RSD 2.01%,n=6),96.71%(RSD1.89%,n=6),97.85%(RSD 2.17%,n=6)。结论:该方法灵敏度高,快速,简便,专属性强,准确和重复性好,为败酱草药材质量控制标准的建立提供科学依据。 Objective:To establish a method for the quantitative determination of hederagenin,oleanolic acid and ursolic acid in Herba Patriniae Simudtanoukly by HPLC.Method:The analysis was performed on Ultimate C18(4.6 mm×250 mm,5 μm) column with mobile phase consisted of methanol-0.2% ammonium acetat water by Gradient elution.The detected wavelength was set at 210 nm.The flow rate was 1.0 mL·min-1 and the column temperature was at 25 ℃.Result: The linearity was good in the ranges of 0.026-0.26,0.082-0.82 and 0.079-0.79 g·L-1 respectively for ederagenin,oaleanolic acid and ursolic acid(r0.999 8).The average recoveries of hederagenin,oleanolic acid and ursolic acid were 100.62%(RSD 2.01%,n=6),96.71%(RSD 1.89%,n=6) and 97.85%(RSD 2.17%,n=6).Conclusion: This method was highly sensitive,rapid,simple,specific,accurate and well represented,which can be used for the quality control of Herba Patriniae.
作者 毛红梅 平欲晖 宗星星 刘玢 MAO Hong-mei,PING Yu-hui,ZONG Xing-xing,LIU Bin(Jiangxi College of Traditional Chinese Medicine,Nanchang 330004,China)
出处 《中国实验方剂学杂志》 CAS 北大核心 2012年第15期89-92,共4页 China Journal of Experimental Traditional Medical Formulae
关键词 败酱草 常春藤皂苷元 齐墩果酸 熊果酸 高效液相色谱法 含量测定 Herba Patriniae hederagenin oleanolic acid ursolic acid HPLC content determination
作者简介 [第一作者]毛红梅,硕士,Tel:13576982640,E-mail:573662247@qq.com [通讯作者]平欲晖,副教授,本科学历,从事药物质量分析研究,Tel:13677090003,E-mail:pingyh@163.com
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