Objective To investigate the distribution and the antimicrobial resistance in clinical isolates of fastidious bacte ria from Tongji Hospital and to guide antibiotic practice. Methods Clinical isolates of Streptococcus pneumoniae, Haernophilus influenzae,and Branhamaceae catarrhalis were collected from 2006 to 2010. The MIC of penicillin was determined by E-test, and other antimicrobial susceptibility was tested by Kirby-Bauer method. Beta-lactamases were detected by nitrocefin disk. Results 503 Streptococcus pneumoniae, 658 Haemophilus influenzae and 142 Branhamaceae catarrhalis were collected. The total rate of PNSSP was 22.5% ,among them 4.7% were PRSP. Streptococcus pneumoniae were high resistant to Clin damycin and Erythromycin, especially those isolated from children. But 90 % of them were susceptible to Moxifloxacin and Levo floxacin,and no strain was resistant to Vancomycin. Haemophilus influenzae were low susceptible to Trimethoprim sulfamethoxazole,accounting for 40.6%;and 60% were susceptible to Ampicillin; but about 85% strains were susceptible to Cefu roxime,Cefotaxime, Azithromycin,Ciprofloxacin and Levofloxacin. The prevalence of beta-lactamase was high in Branhamaceae catarrhalis,about 98.6 %. The resistance rate of all three bacteria was increased to varying degrees year by year. Conclusion The isolates were sensitive to the majority of antibiotics, but more than 30 % strains were non-susceptible to Penicillin, especially Branhamaceae catarrhalis. It was suggested that antibiotics should be chosen according to antimicrobial susceptibility test re sults.
Journal of Huazhong University of Science and Technology（Health Sciences）
Haemophilus in fluenzae
antimicrobial susceptibility test