期刊文献+

常见细菌对紫外线敏感性试验观察 被引量:5

Experimental observation of ultraviolet light sensitivity effect on common bacteria
收藏 分享 导出
摘要 目的试验观察几种常见细菌对紫外线照射的敏感性,为临床使用紫外线消毒灭菌提供理论依据。方法采用细菌悬液接种,将金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、肺炎克雷伯菌、鲍氏不动杆菌、脂肪芽胞杆菌接种到普通琼脂平板,用紫外线垂直照射,并根据紫外线照射时间的不同随机分6组,0s(对照组),10、20、30、60s和90s组为试验组,每组接种5个平板,分别进行照射,观察杀菌效果。结果对照组细菌生长良好,观察组照射10s细菌存活率〈50%,照射90s存活率为0,两组间差异有统计学意义(P〈0.01);不同种细菌敏感性有差异,同种细菌照射时间越长,细菌存活率越低。结论紫外线对常见几种细菌有很好的杀灭效果。 OBJECTIVE To provide a theoretical basis for using UV to disinfect and sterilize in clinical practice by experimental observation on sensitivity of several common bacteria to irradiation by UV. METHODS Using bacterial suspension, Staphylococcus aureus , Escherichia coli , Pseudomonas aeruginosa , Klebsiella pneurnoniae , Acinetobacter baumannii and Bacillus fat were inoculated on ordinary agar plates. Vertical irradiation with ultraviolet was provided. According to ultraviolet irradiation time, they were randomly divided into 6 groups. 0s (The control group), 10s, 20s, 30s, 60s and 90s were the experimental groups. For each group, five plates were inoculated. Irradiation was provided to observe the bactericidal effect. RESULTS The bacteria of control group (0s) grew well. In observation group, after irradiation for 10 s, bacterial survival rate was less than 50%. After irradiation for 90s, survival rate was 0. There was significant difference between the two groups (P〈0.01). Different kinds of bacteria had different sensitivity. For the same type of bacteria, the longer irradiation time was, the lower survival rate was. CONCLUSION UV has excellent effect in killing some common bacteria.
作者 李钟响 吴红映 金美琴 LI Zhong-xiang, WU Hong-ying, JIN Mei-qin (The Second Hospital of Jinhua City, Jinhua, Zhejiang 321016, China)
出处 《中华医院感染学杂志》 CAS CSCD 北大核心 2012年第1期 121-122,共2页 Chinese Journal of Nosocomiology
关键词 紫外线 细菌 消毒时间 效果 Ultraviolet light Bacteria Sterilization time Effect
  • 相关文献

参考文献8

二级参考文献14

共引文献35

同被引文献40

  • 1安涓,尚君兰.口腔医师医院感染的因素及个体防护[J].第三军医大学学报,2006,28(20):2061-2061. 被引量:1
  • 2李香云,刘礼,张长杰,孔瑛.冷光紫外线杀菌效果的实验观察[J].中国现代医学杂志,2006,16(20):3143-3146. 被引量:14
  • 3叶应妩,王毓三,申子瑜.全国I临床检验操作规程[M].3版.南京:东南大学出版社,2006:692-694. 被引量:6
  • 4WS/T368-2012:医院空气净化管理规范[S].北京:中华人民共和国卫生部,2012. 被引量:7
  • 5Barlean L, Danila I, Baleos C, et al. Preventive attitudes towards infection transmission in dental offices in North-East Romania [J]. Rev Med Chir Soc Med Nat lasi, 2012,116(4): 1209 - 1212. 被引量:1
  • 6Rutala W A, Weher D J. Disinfection and sterilization: an overview [J]. Am J Infect Control, 2013, 41(5 Suppl 1, S2 -S5. 被引量:1
  • 7郝世勇,聂华.乙型肝炎病毒表面抗原定量及其临床意义[J].临床血液学杂志:输血与检验版,2013,26(2):141-144. 被引量:1
  • 8中华人民共和国卫生部.医院消毒技术规范:2009版[S].北京:中华人民共和国卫生部,2009. 被引量:1
  • 9Menzies D, Popa J, Hanley J A, et al, Effect of ultraviolet germicidal lights installed in office ventilation systems on workers' health and wellbeing: double-blind multiple crossover trial [J]. Lancet, 2003, 362 (9398) : 1785 -1791. 被引量:1
  • 10Kujundzic E, Matalkah F, Howard C J, et al. UV air cleaners and upper-room air ultraviolet germicidal irradiation for controlling airborne bacteria and fungal spores[J].] Occup Environ Hyg, 2006, 3(10): 536 -546. 被引量:1

引证文献5

二级引证文献13

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈