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杨树幼茎特异表达基因及PsnLAC基因的克隆 被引量:2

Specific Gene Expression in Stem of Populus simonii × P.nigra and Cloning of PsnLAC Gene
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摘要 利用cDNA-AFLP技术对小黑杨茎、叶基因的差异表达进行分析。结果表明,64对引物组合在茎和叶中共扩增出4 192条带,其中差异条带2 275条。通过对茎中特异表达基因的克隆和测序,获得了4条cDNA序列。经Blast-x比对分析表明,它们分别是类伸展蛋白、漆酶、过氧化物酶和细胞壁相关水解酶。进行RT-PCR分析发现,4个基因在茎中的表达水平明显高于叶和根。其中,以漆酶的基因片段为基础进行生物信息学分析和设计引物,用RT-PCR技术克隆到了1 413 bp包含开放阅读编码框的cDNA片段,其开放阅读编码框长度为1 020bp,编码339个氨基酸残基,与来自其他植物漆酶的氨基酸序列具有较高的同源性。 Different expressed genes between stems and leaves of Populus simonii × P.nigra were observed by the cDNA-AFLP method.Result showed that a total of 4 192 fragments were amplified from the cDNA of stems and leaves,and a total of 2 275 fragments were differential displayed genes between stems and leaves.Four fragments from stems were cloned and sequenced.Blast-x analysis showed that the four clones were extension like protein,laccase,peroxidase,cell wall-associated hydrolase.Based on a cDNA fragment of laccase,the 1 413 bp cDNA of laccase in Populus simonii × P.nigra was obtained using RT-PCR,including a 1 020 bp open reading frame,and encoding 339 amino acid residues sharing higher homology with other plants.
作者 吴丽丽 高福玲 王雷 郑威 杨传平 姜廷波 Wu Lili,Gao Fuling,Wang Lei,Zheng Wei,Yang Chuanping,Jiang Tingbo((Key Laboratory of Forest Tree Genetic Improvement and Biotechnology(Northeast Forestry University),Ministry of Education,Harbin 150040,P.R.China)
出处 《东北林业大学学报》 CAS CSCD 北大核心 2011年第4期 5-7,23,共4页 Journal of Northeast Forestry University
基金 黑龙江省科技攻关项目(GA09B201-4)
关键词 小黑杨 RT-PCR CDNA-AFLP 漆酶 Populus simonii × P.nigra RT-PCR cDNA-AFLP Laccase
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