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大鼠活体心肌缺血再灌注损伤模型的建立与综合评估 预览 被引量:19

Establishment and evaluation of rat heart ischemia -reperfusion injury model in vivo
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摘要 目的:构建并评估大鼠活体心肌缺血再灌注损伤(I/R)的实验动物模型. 方法:将清洁级成年SD雄性大鼠72只,随机分为正常组、假结扎组和I/R模型组, 手术的两组全麻下开胸暴露心脏,假结扎组只过线不结扎,模型组使用"U形金属管"进行冠状动脉左前降支结扎,持续心电图监测,并于再灌注2 h与4 h后进行血浆生化﹑心肌组织学的检测.结果:与正常组和假结扎组比较,I/R模型组心电图有明显ST-T改变和再灌注后病理性Q波,再灌注2 h和4 h时存在比较明显的ST-T改变;血标本检测示心肌型肌酸激酶同功酶(CK-MB)、心肌钙蛋白I(cTnI)、一氧化氮(NO)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)水平显著升高(P<0.05或P<0.01),T-SOD水平在2 h和4 h也应激性升高(P<0.05);心肌组织病理学检测显示模型组左心室心肌损伤疤痕明显,且2 h模型组左室心肌存在间隔性梗死区域,4 h模型组心肌组织区域性梗死和梗死面积明显增加;TUNEL法与Bcl-2﹑Bax蛋白实验显示2 h和4 h心肌细胞凋亡数量明显增加;Bcl-2/Bax蛋白表达阳性细胞比值下降显著(P<0.01). 结论:成功建立大鼠活体心肌I/R模型,模型大鼠的心电图、血清学、心梗面积﹑心肌细胞凋亡和心肌组织病理学等发生显著改变. AIM: To establish and evaluate a rat model of heart ischemia - reperfusion injury in vivo. METHODS: Seventy - two male Sprague - Dawley rats weighing(250 ± 50)g were randomly divided into sham operation group(sham), ischemia- reperfusion group(I/R) and normal group. The animals were anesthetized and heparinized. Myocardial ischemia - reperfusion was induced by ligating the left anterior descending coronary artery with "U - shape tube" for 35 min followed by 120 min or 240 min reperfusion in vivo. The heart infarct size was measured by triphenyhetrazolium chloride(TIC) staining. The myocardial cell apoptotic index was determined by the method of terminal deoxynucleotidyl transferase -mediated dUTP - biotin nick - end labeling (TUNEL). Immunohistocbemical method was used to detect the expression of Bcl - 2 and Bax in rat ischemia myocardium. The blood level of MB isoenzyme of creatine kinase ( CK - MB), cardiac troponin I (cTnI), nitric oxide ( NO ), malondialdehyde ( MDA ), total superoxide dismutase ( T - SOD) and glutathione peroxidase(GSH - Px) were detected after reperfusion for 2 h and 4 h. RESULTS: Compared with normal group and sham group, there were obvious changes of ST - T segment and Q wave in the electrocardiogram of I/R group. The blood level of CK - MB, cTnl, NO, MDA and GSH- Px in I/R group increased ( P 〈 0.05,P 〈 0.01 ) after reperfusion for 2 h and 4 h, and the blood level of T - SOD in I/R group after reperfusion for 2 h and 4 h also increased (P 〈 0.05 ). The heart infarct size in L/R group was the largest as compared to other groups. Among these groups, the apoptotic index of I/R group was the highest and the Bcl- 2/Bax ratio in I/R group decreased(P 〈0. 01 ). CONCLU- SION: The rat model of heart ischemia - reperfusion injury in vivo can be successfully established with the "U - shape tube". There are obviously changes of heart infarct size, blood level of CK - MB, cTnI, NO, MDA, T - SOD and GSH -Px, myocardial ap
作者 邓宇珺 符永恒 谭宁 曾红科 单志新 林秋雄 李晓红 董小莉 余细勇 杨敏 谭卫萍 苏健 DENG Yu -jun FU Yong- heng TAN Ning ZENG Hong -ke SHAN Zhi -xin LIN Qiu - xiong , LI Xiao - hong, DONG Xiao - li, YU Xi - yong , YANG Min , TAN Wei - ping, SU Jian( 1 Department of Emergency and Critical Care, 2 Guangdong Cardiovascular Institute, 3Research Center of Medical Sciences, 4Department of Library, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. )
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2011年第2期 412-416,共5页 Chinese Journal of Pathophysiology
基金 [基金项目】广东省科技计划资助项目(No.20088030301166) 广东省自然科学基金资助项目(No.9151008002000002)
关键词 大鼠 再灌注损伤 细胞凋亡 Rats Reperfusion injury Apoptosis
作者简介 通讯作者Tel:020-83827812;E-mail:tanning100@126.com
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