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锰对大鼠生精细胞Caspase-3 mRNA调控及支持细胞波形蛋白表达的影响 被引量:2

Effect of manganese on Caspase-3 mRNA regulation in spermatogenic cell and the expression of vimentin on Sertoli cell in rats
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摘要 目的 探讨锰对大鼠生精细胞Caspase-3 mRNA调控及支持细胞波形蛋白(vimentin)表达的影响.方法 将正常雄性SD大鼠48只,随机分为1个对照组和2个染锰组,给予各组大鼠腹腔注射生理盐水和15mg/kg、30mg/kg氯化锰.分别染锰4周和6周,随机处死各组大鼠8只.应用末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记技术(TUNEL) 、原位杂交法和免疫组织化学链酶亲和素-生物素-过氧化物酶复合物(SABC)法进行检测研究.结果 1. 与空白对照组比较,15mg/kg、30mg/kg染锰组生精细胞凋亡指数(AI)均升高(P〈0.05, P〈0.01),Caspase-3 mRNA阳性细胞率均显著升高(P〈0.01),支持细胞波形蛋白(vimentin)阳性细胞率均显著降低(P〈0.01).2. 染锰剂量相同,染锰6周组与染锰4周组比较,生精细胞AI与Caspase-3 mRNA阳性细胞率均显著升高(P〈0.01),支持细胞vimentin阳性细胞率均显著降低(P〈0.01).3. 染锰时间相同,30mg/kg MnCl2组与15mg/kg MnCl2组比较,生精细胞AI与Caspase-3 mRNA阳性细胞率均显著升高(P〈0.01),支持细胞vimentin阳性细胞率均显著降低(P〈0.01).4. 各组大鼠生精细胞AI和Caspase-3 mRNA阳性细胞率呈正相关(r=0.842, P〈0.01),与支持细胞vimentin阳性细胞率呈负相关(r=-0.859, P〈0.01),各组大鼠生精细胞Caspase-3 mRNA阳性细胞率和支持细胞vimentin阳性细胞率呈负相关(r=-0.975, P〈0.01). 结论染锰大鼠生精细胞Caspase-3 mRNA表达增加导致生精细胞凋亡增加 支持细胞vimentin表达下降 这可能是锰生殖毒性的重要分子机制之一. Objective To determine the effect of manganese on Caspase-3 mRNA regulation in spermatogenic cell and the expression of vimentin on Sertoli cell in rat. Methods Forty-eight male rats were randomly divided into one control group and two manganese chloride( MnC12 ) (15mg/kg,30 mg/kg) groups. After exposed to manganese for 4 and 6 weeks respectively, the apoptosis of spermatogenic cell was examined by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL) technique. The expression of Caspase-3 mRNA in spermatogenic cells was investigated by in situ hybridization, and the expression of vimentin on Sertoli cell of rats was detected by immunohistochemitry(SABC). Results 1. Compared with control groups, AI was increased (P 〈 0. 05 ,P 〈 0. 01 ), the Caspase-3 mRNA-positive-cell rate was increased significantly (P 〈 0. 01 ), the vimentin-positive-cell rate was decreased significantly(P 〈0.01 ) in every manganese exposure groups. 2. Among manganese exposure groups of same dose, AI and the Caspase-3 mRNA-positivecell rate were increased more significantly, but the vimentin-positive-cell rate was decreased more significantly in 6weeks manganese exposure groups than that in 4weeks manganese exposure groups ( P 〈 0. 01 ). 3. Among manganese exposure groups of same time, AI and Caspase-3mRNA-positive-cell rate in 30mg/kg manganese exposure groups were increased more significantly than that in 15mg/kg manganese exposure groups (P 〈 0. 01 ), but the vimentin-positive-cell rate was decreased more significantly in 30 mg/kg manganese exposure groups than that in 15 mg/kg manganese exposure groups (P 〈 0. 01 ). 4. There existed a positive correlation between AI and the Caspase-3 mRNA-positive-cell rate (r = 0. 842, P 〈 0.01) , a negative correlation between AI and vimentin-positive-cell rate ( r = - 0. 859, P 〈 0. 05 ) , and a negative correlation between the Caspase-3 mRNA-positive-cell rate and the vimentin-positive-cell rate ( r = - 0. 975,
作者 才秀莲 王国秀 郭海 CAI Xiu-lian , WANG Guo-xiu,GUO Hai (Department of Histology and Embryology, Zunyi Medical College, Guizhou Zunyi 563000, China)
出处 《解剖学报》 CAS CSCD 北大核心 2010年第3期400-404,共5页 Acta Anatomica Sinica
基金 遵义市科技项目基金资助项目(200707)
关键词 生精细胞 Caspase-3 mRNA 波形蛋白 原位缺口末端标记 原位杂交 免疫组织化学 大鼠 Manganese Spermatogenic cell Caspase-3 mRNA Vimentin TUNEL In situ hybridization Immunohistochemistry Rat
作者简介 才秀莲(1946-),女(汉族),辽宁省锦州市人,教授。通讯作者(To whom correspondence should be addressed)E-mail:lianxiucai@ 163.com Tel: (0852)8609490
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