期刊文献+

甜菜夜蛾烟碱型乙酰胆碱受体β1亚基基因的克隆与序列分析 被引量:1

Molecular cloning and sequence analysis of nicotinic acetylcholine receptor β1 subunit from Spodoptera exigua (Hiibner)
收藏 分享 导出
摘要 烟碱型乙酰胆碱受体是昆虫体内重要的神经受体,同时也是杀虫剂作用靶标。从甜菜夜蛾Spodoptera exigua3龄幼虫体内提取总的RNA,经过反转录,利用RT—PCR获得了烟碱型乙酰胆碱受体6个α和1个β亚基基因的cD-NA序列片段,并利用cDNA末端快速扩增技术(RACE)获得了β亚基基因的cDNA序列全长。该基因命名为SenAChRβ1,其长度为2231个碱基,含有一个1575个碱基的开放读码框,开放读码框编码524个氨基酸残基,预测的分子量为60kDa。推导得到的氨基酸序列与其它昆虫特别是鳞翅目昆虫的烟碱型乙酰胆碱受体β亚基具有高度的同源性,并具有典型的烟碱型乙酰胆碱受体β亚基特征化位点。 Nicotinic acetylcholine receptors (nAChRs) play an important role in excitatory synaptic transmission in insects and are also one of the most important insecticide targets. In our study, sixα subunit and one β subunit gene fragments encoding nAChR subunit protein were obtained isolated from Spodoptera exigua, an economically important pest, rapid amplification of cDNA ends (RACE) and and 3'ends of the mRNA. The gene ( named as the full-length of β subunit gene was obtained through polymerase chain reaction using primers based on the 5' SenAChPq31 ) was 2231 bp, with an open reading frame of 1575 bp encoding a predicted nACh receptor protein of 524 amino acids with a molecular weight of 60 kDa. The predicted nACh receptor protein shared high identity with other several nAChR β subunit proteins, escepically with Lepidoptera insects. Sequence analysis shows that this gene possesses all the characteristics of nAChR gene family.
作者 尚庆利 梁沛 高希武 SHANG Qing-Li, LIANG Pei, GAO Xi-Wu (Department of Entomology, China Agricultural University, Beijing 100193, China)
出处 《环境昆虫学报》 CSCD 北大核心 2009年第3期 197-202,共6页 Natural Enemies of Insects
基金 supported by National Basic Research Program of China ( 2006CB102003 ) National Natural Science Foundation of China ( 30671389, 30530530) National Key Research Program of China for the Eleventh Five-years Plan (2006 BAD08A03 )
关键词 甜菜夜蛾 烟碱型乙酰胆碱受体 基因克隆 序列分析 Spodoptera exigua nicotinic acetylcholine receptor gene clone sequence analysis
作者简介 尚庆利,男,1980年生,博士,主要从事昆虫毒理与害虫抗药性研究,E—mail:shangqingli@gmail.com 通讯作者Author for correspondence,Tel/fax:86-10-62732974;E-mail:gaoxiwu@263.net
  • 相关文献

参考文献23

  • 1Breer H, Sattelle DB, 1987. Molecular properties and functions of insect acetylcholine receptors. J. Insect Physiol, 33 : 771 - 790. 被引量:1
  • 2Corringer PJ, Le Novere N, Changenx JP, 2000. Nicotinic receptors at the amino acid level. Ann. Rev. Pharmacol. Toxicol, 40:431 - 458. 被引量:1
  • 3Feyereisen R, 1995. Molecular biology of insecticide resistance. Toxicol. Lett. , 82/83:83 -90. 被引量:1
  • 4Goff GL, Hamon A, Berge JB, Amichot M, 2005. Resistance to fipronil in Drosophila simulans : influence of two point mutations in the RDL GABA receptor subunit. J. Neurochem. , 92 : 1295 - 1305. 被引量:1
  • 5Huang Y, Williamson MS, Devonshire AL, Windass JD, Lansdell SJ, Millar NS, 1999. Molecular characterization and imidacloprid selectivity of nicotinic acetylcholine receptor subunits from the peach - potato aphid Myzus persicae. J. Neurochem. , 73 : 380 - 389. 被引量:1
  • 6Karlin A, 2002. Emerging structure of the nicotinic acetylcholine receptors. Nat. Rev. Neurosci., 3: 102-114. 被引量:1
  • 7Kumar S, Tamura K, Nei M, 2004. MEGA 3 : integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. , 5 : 150 - 163. 被引量:1
  • 8Kyte J, Doolittle RF, 1982. A simple method for displaying the hydropahtic character of a protein. J. Mol. Biol. , 51 : 463 -499. 被引量:1
  • 9Lansdell SJ, Millar NS, 2000. The influence of nicotinic receptor subunit composition upon agonist, a bungarotoxin and insecticide (imidacloprid) binding affinity. Neuropharmcol., 39:671 -679. 被引量:1
  • 10Leicht W, 1996. Imidacloprid - achloronicotinyl insecticide : biological activity and agricultural significance. Plan. Nach. Bayer, 49:71 - 84. 被引量:1

同被引文献37

  • 1于海龙,肖云,艾静,李霞,宫滨生.毒蕈乙酰胆碱受体亚型关系的研究[J].遗传,2007,29(10):1280-1288. 被引量:4
  • 2Abreu - Villaa Y, Filgueiras CC, Manhaes AC. Developmental aspects of the cholinergic system [J]. Behav. Brain. Res., 2011, 221: 367 - 378. 被引量:1
  • 3Aizono Y, Endo Y, Sattelle DB, et al. Pmthoracicotropic hormone - producing neurosecretory cells in the silkworm, Bomb)~c mori, express a muscarinic acetylcholine receptor[J], Brain Res., 1997, 763:131 - 136. 被引量:1
  • 4Barbara GS, Griinewald B, Paute S, et al. Study of nicotinic acetyleholine receptors on cultured antennal lobe neurones from adult honeybee brains [ J ]. Invert. Neurosci. , 2008, 8 : 19 - 29. 被引量:1
  • 5Blake AD, Anthony NM, Chen HH, et al. Drosophila nervous system muscarinic acetylcholine receptor: transient functional expression and localization by immunocytoehemistry [ J ]. Mol. Pharmacol. 1993, 44:716-724. 被引量:1
  • 6Breer H. Properties of putative nicotinic and muscarinlc cholinergic receptors in the central nervous system of Locusta Migratoria [ J ]. Neurochem. Int. , 1981, 3:43-52. 被引量:1
  • 7Clark J, Meisner S, Torkkeli PH. Immunocytochemical localization of cholineacety transferase and muscarinic Ach receptors in the anlenna during development of the sphinx moth Manduca sexta [J] . Cell Tissue Res. , 2005, 320: 163- 173. 被引量:1
  • 8Collin C, Hauser F, de Valdivia EG, et al. Two types of muscarinic acetylcholine receptors in Drosophila and other arthropods [ J ]. Cell Mol. Life Sci., 2013, DOI 10. 1007/s00018 -013 -1334-0. 被引量:1
  • 9Cordova D, Raymond DV, Sattelle DB, et al. Spatiotemporal calcium signal in a Drosophila melanogaster cell line stably expressing a Drosophila muscarinic acetylcholine receptor[J]. luvert. Neurosci. , 2003, 5 : 19 - 28. 被引量:1
  • 10Eglen RM, Chopin A, Dillon MP, et al. Muscarinic receptor ligands and their therapeutic potential [ J]. Curr. Opiu. Chem. Biol.,1999, 4:426-432. 被引量:1

引证文献1

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈