Using different species of barley as material, the activities of three amylase were determined by the substrate analysis method. It was found that there were significant differences in the activities of α-amylase, β-amylase and limit dextrinase in different barley. The barley contained higher activity should be selected to produce malt. During malt production, the activity of the amylase was inhibited. The activity of the amylase slowly increased in the beginning of the germination course and rapidly enhanced after 2 -3d. Kilning could decrease the activities of three amylase to some extent.