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RP-HPLC法评价北豆根药材质量 预览 被引量:2

Quality assessment of Rhizoma Menispermi by RP-HPLC
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摘要 目的探讨北豆根药材的质量评价标准。方法采用反相高效液相色谱法(RP-HPLC)测定10批北豆根商品药材中蝙蝠葛碱和蝙蝠葛苏林碱的含量。结果北豆根药材中蝙蝠葛碱和蝙蝠葛苏林碱分离完全且呈良好的线性关系,线性范围分别为0.992-4.96μg和1.04-5.20μg;平均加样回收率分别为100.2%(RSD=1.46%)和100.5%(RSD=1.36%)。不同购买地的药材两种生物碱含量差异较大,蝙蝠葛碱为0.092%~0.844%,蝙蝠葛苏林碱为0.322%~1.016%。两种生物碱总含量为0.688%~1.226%,相对较稳定。结论蝙蝠葛碱和蝙蝠葛苏林碱的总含量可作为北豆根药材质量控制指标。 Purpose To assess the quality of the Chinese crude drug Rhizoma Menispermi (the dried rhizome of Menispermum dauricum D C. ). Methods A reversed-phase high performance liquid chromatographic (RP-HPLC) method was used to determine the contents of the two main alkaloids, dauricine and daurisoline, in the Rhizoma Menispermi samples purchased from 10 different districts. Re- sults The two alkaloids could be well separated from other constituents and there was a good linear relationship within the range of 0.992 4.96 μg (r = 0. 999 3) for dauricine and 1.04 - 5.20 μg (r = 0. 999 6) for daurisoline. The average recoveries of dauricine and daurisoline were 100. 2% (RSD = 1.46%) and 100.5% (RSD= 1.36%), respectively. The content of the two alkaloids in the 10 samples varied greatly. It ranged from 0. 092% to 0. 844% for dauricine and 0. 322% - 1. 016% for daurisoline. The total content of the two alkaloids was more stable (0.688 % - 1. 226 ~). Conclusions The total content of dauricine and daurisoline could be used as the marker for quality control of Rhizoma Menispermi.
作者 卢燕 冯举 陈道峰 LU Yan, FENG Ju, CHEN Dao-feng(Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 200032, China)
出处 《复旦学报:医学版》 CAS CSCD 北大核心 2007年第5期 773-775,共3页 Fudan University Journal of Medical Sciences
基金 “九五”国家重点科技攻关项目(99-929-01-31)
关键词 北豆根 蝙蝠葛碱 蝙蝠葛苏林碱 RP-HPLC 质量控制 Rhizoma Menispermi dauricine daurisoline RP-HPLC quality control
作者简介 通讯作者E-mail:dfchen@shmu.edu.cn
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