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口蹄疫病毒受体猪源β1亚基基因的克隆和分子特征 预览 被引量:2

Molecular Cloning and Characteristics of cDNA Encoding Pig β1 Subunit for FMDV Receptor
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摘要 首次从FMDV试验感染康复猪的舌皮和肺组织中克隆到了整联蛋白β1亚基的基因并对其核苷酸序列和推导的氨基酸序列以及蛋白结构进行了分析。猪整联蛋白β1亚基基因的编码区含有2397个核苷酸,编码798个氨基酸残基,含有10个潜在的糖基化位点(NXTX/NXSX),2个表皮生长因子相似结构域和3个半胱氨酸丰富区。其信号肽由20个氨基酸组成,胞外域由708个氨基酸组成,跨膜区由29个氨基酸组成,胞浆域由41个氨基酸组成。猪脚基因与牛、猩猩、猫、犬、人、小鼠和鸡的脚基因的核苷酸序列一致性分别为99.5%,90.0%,91.8%,90.7%,90.2%,86.5%和77.4%,推导的氨基酸序列一致性分别为99.9%,93.9%,97.5%,96.7%,94.2%,92.4%和94.9%。通过生物学软件分析发现β1亚基形成复杂的二、三级结构,其中1~20位、729~757位氨基酸区段疏水性较强,分别是该亚基的信号肽和跨膜区。为进一步深入研究FMDV嗜性、与宿主细胞的相互作用、病毒的侵入机制等问题奠定了基础。 In this study, we first molecularly cloned pig integrin β1 from RNA of the tongue and lung of recovered pig infected experimentally with foot-and-mouth-disease virus (FMDV), and compared with the β1 gene of other animals available in GenBank at nucleotide and amino acid leves. The 2 397 bp cDNA of pig integrin β1 encodes a polypeptide of 798 amino acids consisting of 10 potential N-linked glycosylation sites(NX3X/NXSX) ,2 EGF-like domains and 3 cysteine-rich regions. Pig integrin β1 subunit has a 20-residue putative signal peptide, a 708-residue ectodomain, a 29-residue transmembrane domain,and a 41-residue cytoplasmic domain. The nucleotide sequence similarity of integrin pl between bovine, chimpanzee, cat, dog, human, mouse, chicken is 99.5 %, 90.0 %, 91.8 %, 90.7 %, 90.2 %, 86.5 % and 77.4 %, and the amino acid sequence similarity is 99.9% ,93.9% ,97.5% ,96.7% ,94.2% ,92.4% and 94.9% respectively. Hydrophobicity analysis of the polypeptide revealed two hydrophobic domains, the signal peptide (1-20AA)and transmembrane domain (729-757AA). This study will lay a foundation for understanding the interactions of FMDV with receptors.
作者 高闪电 独军政 常惠芸 周建华 谢庆阁 GAO Shan-dian, DU Jun-zheng,CHANG Hui-yun, ZHOU Jian-hua,XIE Qing-ge (Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China)
出处 《华北农学报》 CSCD 北大核心 2007年第6期 14-18,共5页 Acta Agriculturae Boreali-Sinica
基金 国家重点基础研究发展计划“973”项目资助(2005CB523201)
关键词 口蹄疫病毒 受体 猪整联蛋白β1基因 分子特征 FMDV Receptor Pig integrin β1 cDNA Molecular characteristics
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参考文献13

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