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椭圆食粉螨线粒体DNA COⅠ基因片段序列分析 预览 被引量:8

Analysis of Mitochondrial DNA COⅠ Gene Fragment of Aleuroglyphus ovatus
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摘要 采用苯酚-氯仿-异戊醇抽提法提取了采自江西南昌和广州潮州2个地理种群的椭圆食粉螨Aleuroglyphus ovatus Troupeau,1878基因组DNA。以相应引物对椭圆食粉螨线粒体DNA细胞色素氧化酶Ⅰ亚基基因(mtDNA COⅠ)进行PCR扩增,直接测序,得到379bp的碱基片断(国际基因库索引号EF527825),其碱基序列A、C、G、T含量分别为71bp(18.73%)、53bp(13.98%)、89bp(23.48%)、166bp(43.80%);江西南昌和广州潮州2个地理种群间的椭圆食粉螨mtDNA COⅠ基因片段完全一致,未发现地理差异。 The genomie DNA ofAleuroglyphus ovatus was extracted by phenol : Chloroform: isoamyl alcohol, The specimens in the present study were collected from NanChang in JiangXi Province and ChaoZhou in GuangZhou Province. In our research, mitoehondrial eytoehrome oxidase subunit Ⅰ ( CO Ⅰ ) gene fragment of Aleuroglyphus ovatus was successfully amplified and sequenced, 379 hp sequences of mtDNA CO Ⅰ were obtained (accession no. EF527825 in GenBank). The contents of A,C,G and T were 71 bp (18.73 %) ,53 bp (13.98 %) ,89 bp (23.48 %) ,166 bp (43.80 %), respectively. There is no difference in the CO Ⅰ gene fragment ofAleuroglyphus ovatus between the two different regions.
作者 吴太葆 夏斌 邹志文 桂梓 朱志民 WU Tai-Bao, XIA Bin, ZOU Zhi-Wen, GUI Zi, ZHU Zhi-Min (College of Life Science, Nanchang University, Nanchang 330031,China)
出处 《蛛形学报》 2007年第2期 79-82,共4页 Acta Arachnologica Sinica
基金 江西省自然科学基金项目(530070) 江西省教育厅科研项目
关键词 椭圆食粉螨 线粒体DNA CO Ⅰ基因 Aleuroglyphus ovatus mitoehondrial DNA CO Ⅰ gene
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参考文献6

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