Objective： To observe the thermogenesis induced with isoprenaline in rats. Method： After isoprenaline was administrated intravenously, the core and skin temperatures of female rats at thermoneutrality （28 ℃ as the circumstance temperature） were observed for 180 minutes, the peak of temperatures and the area under temperature curve were used to calculate its ED50 with the software Prism-4 respectively. Result： The ED50 of isoprenaline from core temperature as the peak and the area were 0.277 mg·kg^-1 and 0. 106 mg·kg^-1 respectively in female rats. The skin temperatures at the center of chest was always positive correlative with the core temperatures, and the skin temperatures at the end of extremities was some positive correlative with the core temperatures. Conclusion： Since the equal ED50 of thermogenesis induced with isoprenaline located at the range of clinic used dose, the clinic actions of isoprenaline should involve its thermogenesis.