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胰腺癌组织中淋巴管生成的机制及意义胰腺癌组织中淋巴管生成的机制及意义胰腺癌组织中淋巴管生成的机制及意义胰腺癌组织中淋巴管生成的机制及意义 被引量:6

Expressions of VEGF-C, D/VEGFR-3 in pancreatic cancer and their clinical significance
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摘要 目的:探讨胰腺癌组织中血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C)、血管内皮生长因子D(vascular endothelial growth factor-D,VEGF-D)与微淋巴管密度(microvessel vessd density,MLVD)、微血管密度(microvessel density,MVD)及淋巴结转移之间的相关关系,阐明癌周淋巴管增生的机制及意义.方法:免疫组化检测30例胰腺癌组织中VEGF-C、VEGF- D、血管内皮生长因子受体3(vascular endothelial growth factor receptor-3, VEGFR-3)(MLVD)以及CD34(MVD)蛋白的表达,RT-PCR检测VEGF-A、VEGF-C、VEGF-D mRNA在30例胰腺癌新鲜组织中的表达.结果: VEGF-C、VEGF-D蛋白阳性率分别为73%(22/30)和57%(17/30),肿瘤周边部位显著高于肿瘤中心部位,差异有统计学意义,P <0.05; 其表达与肿瘤的部位、分化程度以及组织学类型无关,而与肿瘤的TNM分期有关,Ⅲ、Ⅳ期显著高于Ⅰ、Ⅱ期.在VEGF-C蛋白阳性组,MVD高于阴性组, P=0.047 2,MLVD亦高于阴性组,P<0.01,淋巴结转移增多,P=0.031 8;VEGF- D蛋白阳性组与阴性组相比MVD无变化, P=0.07,MLVD高于阴性组, P<0.01,淋巴结转移增加,P=0.017 9.结论: VEGF-C参与胰腺癌血管生成和淋巴管生成的调控,VEGF-D只参与淋巴管生成的调控.VEGF-C和VEGF-D诱导胰腺癌淋巴管生成,促进肿瘤细胞淋巴道转移. OBJECTIVE: To investigate the relationship between VEGF-C, VEGF-D, MLVD, MVD and lymph node metastasis in pancreatic cancer, and elucidate the mechanism and significance of the cancerous peripheral tissue lymphatic. METHODS: The expressions of VEGF-C, VEGF-D, MLVD,MVD were assayed by means of immunohistochemistry in 30 patients with pancreatic carcinoma. RESULTS: The positive rates of VEGF-C, VEGF-D were 73% (22/30), 57% (17/30) respectively in pancreatic cancer. The expressions of VEGF-C and VEGF-D in cancerous invasive edge were significantely higher than those in the center of cancerous tissues (P〈0.05). There was no correlation between the expressions of VEGF-C, VEGF-D and the site, differentiation, histology types. Ⅲ, Ⅳ stages of pancreatic cancer showed strong expressions of VEGF-C,VEGF-D than Ⅰ , Ⅱ stages of pancreatic (P〈0.01). The MVD, MLVD and positive lymph node in positive VEGF-C group were higher than in the negative group. The MLVD and positive lymph node in positive VEGF-D group were higher than those in the negative group. CONCLUSIONS: VEGF-C participates in the regulation of angiogenisis and lymphangiogenisis in pancreatic cancer, but VEGF-D only participates in the regulation of lymphangiogenisis. VEGF-C and VEGF-D induce lymphangiogenisis in pancreatic cancer and promote the tumor cell lymph metastasis.
作者 胡安国 王东 毛新彦 张波 衣龙海 李胜 张毅 周武元 HUAn-guo, WANG Dong, MAO Xin-yan, ZHANG Bo, YI Long-hai, LI Sheng, ZHANG Yi, ZHOUWu-yuan(1. Department of General Surgery, Second Affiliated Hospital, Shandong University of Traditional Chinese Medince, Jinan 250001, P. R. China; 2. Department of Hepatobiliary Surgery, Shandong Tumor Hospital, J inan 250117, P. R. China)
出处 《中华肿瘤防治杂志》 CAS 2007年第4期 254-257,共4页 Chinese Journal of Cancer Prevention and Treatment
基金 国家自然科学基金(30571712) 山东省优秀中青年科学家科研奖励基金(2005BS03003) 山东省卫生厅资助项目(2005-169)
关键词 胰腺肿瘤/病理学 血管内皮生长因子C 血管内皮生长因子D 淋巴管生成 淋巴转移 pancreatic neoplasms/pathology vascular endothelial growth factor C~ vascular endothelial growth fac-tor D lymphangiogenesis lymphatic netastasis
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  • 1Valtola R,Salven P,Heikkila P,et al.VEGFR-3 and its ligand VEGF-C are associated with angiogenesis in breast cancer[J].Am J Pathol,1999,154(5):1381-1390. 被引量:1
  • 2Ishikawa M,Kitayama J,Kazama S,et al.Expression of vascular endothelial growth factor (VEGF)-C in preoperative biopsy specimens and metastatic foci of regional lymph nodes in submucosal gastric carcinoma[J].World J Surg Oncol,2005,3(1):2. 被引量:1
  • 3Jeltsch M,Kaipainen A,Joukov V,et al.Hyperplasia of lymphatic vessels in VEGF-C tramsgenic mice[J].Science,1997,276(5317):1423-1425. 被引量:1
  • 4Padera T P,Kadambi A,Tomaso T,et al.Lymphatic metastasis in the absence of function intratumor lymphatic[J].Science,2002,296(5574):1883-1887. 被引量:1
  • 5Onogawa S,Kitadai Y,Tanaka S,et al.Expression of VEGF-C and VEGF-D at the invasive edge correlates with lymph node metastasis and prognosis of patients with colorectal carcinoma[J].Cancer Sci,2004,95(1):32-39. 被引量:1
  • 6Niki T,Iba S,Tokunou M,et al.Expression of vascular endothelial growth factors A,B,C and D and their relationships to lymph node status in lung adenocarcinoma[J].Clin cancer Res,2000,6(6):2431-2439. 被引量:1
  • 7Hung C J,Ginzinger D G,Zarneger R,et al.Expression of vascular endothelial growth factor C in benign and malignant thyroid tumors[J].J Clin Endocrinol Meta,2003,88(8):3694-3699. 被引量:1
  • 8Nakamura Y,Yasuoka H,TSujimoto M,et al.Flt4positive vessel density correlates with vascular endothelial growth factor-d expression,nodal states,and prognosis in breast cancer[J].Clin Cancer Res,2003,9(14):5313-5317. 被引量:1

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