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天山雪莲叶片全长cDNA文库的构建 预览 被引量:11

Construction of Full-length cDNA Library from the Saussurea involucrata Kar. et Kir Laminae
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摘要 以天山雪莲(Saussurea involucrata Kar.et Kir)幼嫩叶片为研究材料,利用Creator^TM SMART^TM cDNA Library Construction技术构建了天山雪莲叶片全长cDNA文库,原始文库滴度达3.93×10^5cfu/mL,文库插入片段多在1000bp左右。随机挑取10个克隆进行测序,通过生物信息学初步分析表明,100%为全长cDNA,大部分可以在GenBank中找到同源序列。天山雪莲叶片全长cDNA文库的构建为雪莲基因资源保存和植物抗寒机理的研究奠定了基础。 A full-length eDNA Library from the Saussurea involucrata Kar. et Kir laminae was con structed by using CreatorTM SMARTTM eDNA Library Construction Protocol . The titer of eDNA library was estimated as 3.93×10s cfu/mL, which was about 500 times coverage of gene expressional profile, and the inserted fragments in the library are about 1000 bp length. Ten clones were randomly selected to sequence and bioinformatics analysis was carried out, all of the sequences were full length eDNA,and most of the sequences were homologous to the genes known in GenBank. This full-length eDNA Library provides a important tool for the study of the cold resistence mechanisms of the Saussurea involucrata Kar. et Kit.
作者 祝建波 刘海亮 王重 周鹏 ZHU Jian-bo, LIU Hai-liang, WANG Zhong, ZHOU Peng (1. Key Laboratory of Oasis Ecology Agriculture of Xinjiang Bing tuan, Shihezi Xinjiang 832003,China;2. Biology College of Shihezi University, Xinjiang 832003,China;3. National Key Biotechnology Laboratory for Tropical Crops,CATAS,Haikou 571101, China)
出处 《西北农业学报》 CSCD 北大核心 2006年第6期 170-173,共4页 Acta Agriculturae Boreali-occidentalis Sinica
基金 兵团科技攻关资助项目(NKB025DXNK01SW).
关键词 天山雪莲 试管苗 全长CDNA文库 Saussurea involucrata Kar. et Kir Plantlet Full-length cDNA library
作者简介 祝建波,男,副研究员,作物遗传育种博士生。E-mail:z-j-b@tom.com,通讯地址;新疆石河子大学生物技术中心,邮编:832003 通讯作者。
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