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Bacillus subtilis fmbR抗菌物质的分离和鉴定 预览 被引量:23

Isolation and Identification of the Antimicrobial Substance Produced by Bacillus subtilis fmbR
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摘要 【目的】对Bacillus subtilis fmbR产生的抗菌物质进行分离和鉴定研究,以确定抗菌物质的组成和结构。【方法】采用HPLC和TLC层析对Bacillus subtilis fmbR抗菌物质进行分离纯化,通过ESI-MS和MALDI-MS分析对抗茵物质的组成和结构进行初步鉴定。【结果】BPLC层析表明了Bacillus subtilis fmbR抗茵物质含有保留时间与surfactin相似的成分。TLC层析和原位酸解证明了Bacillus subtilis fmbR抗菌物质含有闭合肽键类的物质,其中之一为相对迁移率Rf与标样surfactin相近的组分。采用ESI-MS分析检测到Bacillus subtilis fmbR抗菌物质含有分子量与surfactinh相同的m/z1009.1、m/z1023.2和m/z1037。0等3种同系物;通过MALDI-MS分析获得[M+H]’为m/z3403.95抗菌物质,该物质分子量与Bacillus subtilis168产生的细菌素subtilosin的m/z3403.3相同。【结论】Bacillus subtilis fmbR抗菌物质由C13~C15的3种surfactinA同系物和一种羊毛硫抗生素subtilosin组成。 [Objective] Isolation and identification of the antimicrobial substances produced by Bacillus subtilis fmbR was studied in this paper. The study aimed to determine the structure and component of the antimicrobial substances. [Method] With HPLC and TLC methods, the antimicrobial substances from Bacillus subtilis fmbR were isolated and purified. Through ESI-MS and MALDI-MS analysis, the structure and component of the antimicrobial substances were identified. [Result] HPLC showed that one of the antimicrobial substances had the similar retention time similar to surfactin. In addition, the antimicrobial substances were proved to include the closed circle peptide band by TLC, and one of them had the migrating rate similar to surfactin. Furthermore, ESI-MS analysis indicated that Bacillus subtilis fmbR could produce three homologues of surfactin, such as m/z1009.1, m/z1023.2 and m/z1037.0. While other antimicrobial substance with the [M+H]+ ion peak at m/z 3403.95 from Bacillus subtilis fmbR, which is very similar to molecular mass of subtilosin produced by Bacillus subtilis 168 was detected by MALDI-TOF-MS analysis [Conclusion] The antimicrobial substances from Bacillus subtilis fmbR were composed of three homologues of surfactinA (C 13-C 15) and a lantibiotics named subtilosin
作者 别小妹 陆兆新 吕凤霞 赵海珍 杨胜远 孙力军 BIE Xiao-mei, LU Zhao-xin, Lü Feng-xia, ZHAO Hai-zhen, YANG Sheng-yuan, SUN Li-jun ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095)
出处 《中国农业科学》 CAS CSCD 北大核心 2006年第11期 2327-2334,共8页 Scientia Agricultura Sinica
基金 江苏省高新技术项目(BG2003311)资助
关键词 枯草芽孢杆菌(Bacillus SUBTILIS fmbR) 抗菌物质 分离 鉴定 Bacillus subtilis fmbR Antimicrobial substance Isolation Identification
作者简介 别小妹(1964-),女,湖北秭归人,副教授,博士,研究方向为食品微生物和生物技术。Tel:13851785267:E-mail:bxan43@yahoo.com.cn。 通讯作者陆兆新(1957-),男,江苏无锡人,教授,博士,研究方向为食品微生物和生物技术。Tel:025-84396583:E-mail:fmb@njau.edu.cn
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