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流感嗜血杆菌耐药基因及耐药机制分析 预览 被引量:7

Detection of Ampicillin - Resistant Genes and Studies on the Molecular Mechanisms of Ampicillin - Resistant Haemophilus Influenzae
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摘要 目的从分子水平了解南京地区儿童流感嗜血杆菌(Hi)对氨苄西林的耐药状况。方法对158株Hi临床分离株进行β-内酰胺酶检测,PCR扩增β-内酰胺酶的TEM及ROB编码基因,并克隆到T载体中作核酸序列测定与分析。结果本地区儿童感染Hi对氨苄西林的耐药率为41.77%。β-内酰胺酶阳性率为40.51%。TEM基因阳性89株,ROB阳性基因1株。63株耐氨苄西林且β-内酰胺酶阳性,耐药性主要是由于产生β-内酰胺酶和TEM基因。2株耐氨苄西林且β-内酰胺酶阴性。1株β-内酰胺酶阳性,但未检测到TEM或ROB基因。结论本地区儿童感染Hi对氨苄西林的耐约情况不容乐观,耐药机制主要是产生β-内酰胺酶,且以TEM型为主。 Objective To study the molecular mechanisms of ampicillin-resistant haemophilus influenzae (Hi) in Nanjing. Methods One hundred and fifty - eight strains of Hi isolated from children wcrc collected to detect bata-lactamase. TEM and ROB bata-lactamase genes were detected by polymerase chain reaction (PCR), and cloned into T vector for sequencing. Results The rate of ampicillin resistance was 41.77% in Hi isolated from children in Nanjing, 40.51% was found to be bata - lactamase production. Eighty - nine strain were TEM positives, 1 strain was ROB positive, 63 strains bata - lactamase positive ampicillin-resistant Hi were identified. The resistance mechanism of ampicillin resistant Hi was production of bata - lactamase, mainly TEM - type enzyme. Two bata-lactamase negative ampicillin - resistant Hi were identified, predicts the other mechanisms of ampicillin-resistant Hi was occuered yet. One strain of non - TEM - type, and non - ROB - type bata - lactamase - producing Hi was identified. Conclusions Amplcillin - resistant in Hi isolated from children in this region is challenging. TEM bata - lactamase is the principal mechanism of ampicillin - resistant of Hi.
作者 钟天鹰 王惠云 谈华 陈倩 胡正 糜祖煌 迟富丽 ZHONG Tian-ying, WANG Hui-yun, TAN Hua, CHEN Qian, HU Zheng, MI Zu-huang, CHI Fu-li (1a. Department of Clinical Laboratory; 1b. Pediatric Research Institute, the Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China; 2. Wuxi Clone Genetic Institute, Wuxi 214026, China)
出处 《实用儿科临床杂志》 CAS CSCD 北大核心 2006年第10期 589-591,共3页 Journal of Applied Clinical Pediatrics
基金 南京市卫生局课题资助(YKK0436)
关键词 儿童 流感嗜血杆菌 Β-内酰胺酶 基因 耐药基因 耐药机制 child haemophilus influenzae bata - lactamase gene
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