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硫化氢供体对实验性高肺血流性肺动脉高压及内源性一氧化碳/血红素氧合酶体系的影响 预览 被引量:9

Impact of hydrogen sulfide donor on experimental pulmonary hypertension induced by high pulmonary flow and endogenous carbon monoxide/heme oxygenase pathway
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摘要 目的:探讨硫化氢供体——硫氢化钠(sodium hydrosulfide,NaHS)对大鼠高肺血流性肺动脉高压及内源性一氧化碳(carbonmonoxide,CO)/血红素氧合酶(hemeoxygenase,HO)体系的影响。方法:将32只雄性SD大鼠随机分为分流组(n=8)、分流+NaHS组(n=8)、假手术组(n=8)和假手术+NaHS组(n=8)。对分流组和分流+NaHS组大鼠行腹主动脉-下腔静脉穿刺建立高肺血流动物模型。分流11周后,测定肺动脉收缩压(systolic pulmonary artery pressure,SPAP)和肺组织CO含量;光镜下计算肺血管中肌型动脉(muscularized artery,MA)、部分肌型动脉(partially muscularized artery,PMA)和非肌型动脉(non-muscularized artery,NMA)百分比,以及肌型动脉的相对中膜厚度(relative medial thickness,RMT)和相对中膜面积(relative medial areas,RMA);电镜下观察其超微结构变化;应用蛋白质免疫印迹技术(Western blot)检测大鼠肺组织HO-1含量。结果:术后11周,分流组与假手术组比较,大鼠SPAP增高48.6%;肺MA和PMA百分比分别升高74.2%和90.9%,NMA百分比降低32.2%,在中型MA和小型MA中RMT分别升高83.6%和86.9%,RMA分别升高74.4%和39.9%;大鼠肺腺泡水平肌型动脉内皮细胞变性明显,内外弹力层不规则,平滑肌细胞体积增大,呈合成表型;大鼠肺组织CO和HO-1含量变化不明显。而分流+NaHS组与分流组比较,大鼠SPAP降低19.8%;肺MA和PMA百分比分别降低14.4%和12.2%,NMA百分比升高13.9%,在中型MA和小型MA中RMT分别升高16.2%和14.3%,RMA分别升高26.9%和14.3%;大鼠肺腺泡水平肌型动脉内皮细胞变性减轻;内弹力层比较规则;平滑肌细胞呈收缩表型;大鼠肺组织CO含量升高25.5%,HO-1蛋白表达升高114.3%。结论:NaHS可以缓解高肺血流性肺动脉高压形成和肺血管结构重建,其� Objective: To explore the possible impact of hydrogen sulfide donor-+NaHS ( sodium hydrosulfide) on experimental pulmonary artery structural remodeling induced by high pulmonary flow and endogenous carbon monoxide/heme oxygenase pathway. Methods: Thirty-two male SD rats were randomly divided into shunt group ( n = 8 ), shunt + +NaHS group ( n = 8 ), sham group ( n = 8 ) and sham + +NaHS group ( n = 8 ). Rats in shunt group and shunt + NailS group were subjected to an abdominal aorta-inferior vena eava shunt to create an animal model of high pulmonary flow. In the sham group and sham + Nails group, rats experienced the same experimental processes except the shunting procedure. After 11 weeks of operation, systolic pulmonary artery pressure (SPAP) was detected using a right cardiac catheterization procedure. The percentage of muscularized artery ( MA), partly muscularized artery (PMA) and non-muscularized artery (NMA) was calculated. Relative medial thickness (RMT) and relative medial area (RMA) of MA were observed under optical-microscope, respectively. The ultra-structure of pulmonary arteries was observed under electro-microscope. Lung tissue carbon monoxide (CO) was measured. Western-blot was used to analyze lung tissue heme oxygenase ( HO-1 ) protein expression. Resuits: After 11 weeks of shunt, compared with sham group, SPAP in shunt group increased by 48.6%.The percentages of MA and PMA increased by 74.2% and 90.9%, but the percentage of NMA decreased 32.2%, respectively, in rats of shunt group as compared with that of sham group. In contrast to those in sham group, RMT and RMA in median MA and small MA of rats in shunt group increased by 83.6%, 86.9%, and 74.4%, 39.9%, respectively. Ultra-structural changes in rats of shunt group showed that endothelial cells were swollen and denatured, inner elastic membrane became irregular, and smooth muscle cells became synthetic phenotype. The sham and shunt groups did not differ significantly in C
作者 李晓惠 杜军保 丁亚光 金红芳 卜定方 汤秀英 唐朝枢 LI Xiao-hui ,DU Jun-bao ,DING Ya-guang ,JIN Hong-fang , BU Ding-fang ,TANG Xiu-ying ,TANG Chao-shu ( Peking University First Hospital 1. Department of Pediatrics, 2. Laboratory Center,3. Laboratory of Electron Microscopy, 4. Institute of Cardiovascular Sciences, Beijing 100034, China)
出处 《北京大学学报:医学版》 CAS CSCD 北大核心 2006年第2期 135-139,共5页 Journal of Peking University:Health Sciences
基金 国家杰出青年科学基金(30425010)、国家重点基础研究发展规划项目(G2000056905)和北京市自然科学基金(7052043)资助
关键词 硫化氢 局部血流 一氧化碳 血红素氧化酶(脱环) Hydrogen sulfide Regional blood flow Lung Carbon monoxide Heme oxygenase ( decyclizing )
作者简介 Corresponding author's e-mail, junbaodul@ 126. com
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参考文献15

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