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苦昧酸-天狼猩红偏振光法对瘢痕动物模型的鉴定书 预览 被引量:4

Identification of scar animal models with method of picric-sirius red polarized light
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摘要 背景:在瘢痕研究中急需稳定可靠的实验动物模型。目的:运用组织学方法对裸鼠瘢痕动物模型进行鉴定,确定最佳使用时机。设计:随机对照的重复测量设计。单位:中山大学附属第一医院烧伤科。材料:实验于2004—01/03在中山大学医学院动物实验中心完成。15只4-6周龄裸鼠(性别随机,体质量15~25g)由中山大学医学院实验动物中心提供;增生性瘢痕来自烧伤愈后、瘢痕增生半年之患者手术切除标本。方法:于裸鼠背侧移植人增生性瘢痕,建立疤痕动物模型;移植后4周起,每周处死5只实验动物,取移植物,100g/L甲醛固定标本,持续3周。苦味酸-天狼猩红染色偏振光检测移植物及临床取材,观察组织特点。主要观察指标:①苦味酸-天狼猩红染色偏振光法读片结果。②计算机图像分析结果。结果:①苦味酸-天狼猩红染色偏振光法读片结果:各时段组移植物在偏振光下呈现相同的以黄、红粗大纤维为主、疏网状绿色纤细纤维散在分布的特点。②计算机图像分析结果:临床病理性增生性瘢痕中,Ⅰ型胶原约占74%,Ⅲ型胶原约占26%;4~6周移植物中,Ⅰ型胶原含量分别为(74.52±0.47)%,(74.43±0.53)%,(74.69±0.63)%;Ⅲ型胶原分别约为(25.48±0.47)%,(25.57±0.53)%,(25.31±0.63)%,差异不显著(P〉0.05)。结论:在实验所设计时段中,移植物与临床取材特性一致,符合增生性瘢痕特点。苦味酸-天狼猩红染色偏振光检测瘢痕组织胶原是对增生性瘢痕组织测定的简便、有效手段;运用裸鼠建立瘢痕动物模型是行之有效的、稳定的。 BACKGROUND: Stable reliable experimental animal models are needed urgently in scar research. OBJECTIVE: Scar animal models of nude mice are evaluated with histological method to define optimal opportunity for using. DESIGN: Randomizly controlled and repetitively measured design. SETTING: Department of Burn, First Affiliated Hospital, Sun Yat-sen University. MATERIALS: The experiment was conducted at the Center for Animal Experiment, Medical College, Sun Yat-sen University between January 2004 and March 2004. Fifteen nude mice aged 4-6 weeks were provided by Center for Animal Experiment, Medical College, Sun Yat-sen University (of either gender with body mass of 15-25 g). Hyperplastic scar was gained from samples of exairesis in patients with burn after healing which is hyperplastic scar for half a year. METHODS: Human hyperplastic scar was grafted at dorsa of nude mice to establish scar animal models. After graft for four weeks, 5 experimental animals were killed every week, and grafts were gained. 100 g/L formalin was used to fix samples for 3 weeks. Picric-sirius red polarized light method was used to detect the graft and clinical materials, and histological feature was ohserved. MAIN OUTCOME MEASURES: ①Results of film reading of picric-sirius rod polarized light method. ②Analytic result of computer image. RESULTS:①Results of film reading of picric-sirius red polarized light method: The grafts showed the same feature of diffused distribution of mainly yellow and red thick fiber with thin-mesh green fiber under polarized light in every time segment group. ②Analytic result of computer image: In clinicopathological hyperplastic scar, type Ⅰ collagen was about 74%; type Ⅲ collagen accounted for about 26%. In the graft from 4-6 weeks, the contents of type Ⅰ collagen were (74.52±0.47)% , (74.43±0.53)% , (74.69±0.63)%, respectively; The contents of type Ⅲ were (25.48±0.47)%, (25.57±0.53)%, (25.31±0.63)%, respectively, which had insignificant differe
作者 朱斌 朱家源 张涛 唐冰 李新强 郑树森 陈东 张伟 李爽 Zhu Bin, Zhu Jia-yuan, Zhang Tao, Tang Bing, Li Xinoqiang, Zheng Shu-sen, Chen Dong, Zhang Wei, Li Shuang(1.Department of Burn, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; 2.Department of Burn, Guangzhou Municipal Red Cross Hospital, Guangzhou 510220, Guangdong Province, China; 3.Department of Burn, People's Hospital of Baoan District of Shenzhen City, Shenzhen 518101, Guangdong Province, China; 4.Room of Image Analysis, Medical College, Sun Yat-sen University, Guangzhou 510080, Guarigdong Province, China)
出处 《中国临床康复》 CSCD 北大核心 2006年第12期 182-183,F0003,共3页 Chinese Journal of Clinical Rehabilitation
基金 国家自然科学基金(39770842).
作者简介 朱斌,男,1967年生,上海市人,汉族,2004年中山大学毕业,硕士,主治医师,讲师,主要从事瘢痕防治、慢性溃疡方面的研究。 通迅作者:朱家源,中山大学附属第一医院烧伤科,广东省广州市510080
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参考文献3

  • 1Zhang J,He JW,W ang TL,etal.Identification Collagen type I andⅢwith picric-sirius red polarized lightmethod[].Chinese Journal of Pathology.1996 被引量:1
  • 2LiXJ,LeiT,Gao JH,etal.Testing collagen in hypertrophic scarwith picric-sirius red polarized lightmethod[].DiyiJunyiDaxue Xuebao.2002 被引量:1
  • 3Sato M,Ishikawa O,M iyachiY.Distinctpatterns ofcollagen gene expression are seen in norm al and keloid fibroblasts grown in three-dimensional culture[].Br J Derm atol.1998 被引量:1

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