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二苯乙烯苷对海马神经元细胞缺血性损伤模型大鼠的保护作用研究 预览 被引量:19

Protective Effect of Diphenyl Ethylene Glycoside on Hippocampal Neuron Cells Ischemic Injury Model Rats
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摘要 目的:研究二苯乙烯苷(TSG)对大鼠神经细胞缺血性损伤的保护作用。方法:应用连二亚硫酸钠建立大鼠海马神经元细胞缺血性损伤模型,以四甲基偶氮唑盐(MTT)微量比色法及乳酸脱氢酶(LDH)漏出率测定法考察TSG对拟缺血性脑损伤的拮抗作用。结果:拟缺血性脑损伤可引起神经元细胞发生明显的损伤性变化,细胞死亡率升高,MTT值明显下降,LDH漏出增加:应用TSG后可减少LDH漏出,增加MTT值,降低细胞死亡率。结论:TSG对神经细胞缺血性损伤模型大鼠具有明显的保护作用。 OBJECTIVE: To investigate the protective effects of diphenyl ethylene glycoside(TSG) on hippocampal neuron cells ischemic injury model rats. METHODS: Hippocampal nerve cells ischemic injury rats' model was established with sodium dithionite, antagonism of TSG on ischemic cerebral injury was determined by methyl thiazolyl tetrazolium (MTT) microcolorimetry and lactate dehydrogenase(LDH) efflux assay. RESULTS: Pseudo-ischemic cerebral injury could cause significant injury to neurons with elevated cell death rate, significantly decreased MTT value and increased LDH efflux rate. The application of TSG could lead in significantly decreased LDH efflux rate, elevated MTT value and reduced cell death rate. CONCLUSION :TSG has a significant protective effect on nerve cells ischemic injury model rats.
作者 李雅莉 赵玲 李林 LI Yali, ZHAO Ling, LI Lin(Pharmaceutical Research Laboratory, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China)
出处 《中国药房》 CAS CSCD 北大核心 2006年第1期 12-14,共3页 China Pharmacy
基金 国家重点基础研究发展计划“973”项目(2003CB517104):首都医学发展基金项目(首发03Ⅲ21) 北京市自然科学基金重大项目(7050001) 北京市科技计划项目(D0204003000031) 北京市科技新星计划项目(H020821390190) 首都医学发展基金项目(2002-3002)
关键词 二苯乙烯苷 海马神经元细胞 缺血性损伤 连二亚硫酸钠 保护作用 Diphenyl ethylene glycoside Hippocampal nerve cell Ischemic injury Sodium dithionite Protection effect
作者简介 技师。研究方向:神经药理学。电话:010-63013355-2881。E-mail:y11200014@yahoo.com.cn 通讯作者。教授,研究员,博士研究生导师。研究方向:神经药理学。电话:010—63132779;E—mail:linli97@hotmail.com
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