A total of 208 SSR primers were used to detect 42 major parents of hybrid rice that were widely popularized in Sichuan Province. Polymorphism were detected with 123 pairs of the primers accounting for 59.13% of the total. Fingerprinting maps of these 42 widely used parents were constructed. The results showed significantly higher genetic diversity on chromosomes 9 and 10 and considerably lower diversity on chromosome 12. The genetic diversity of these materials was limited, but it was remote correspondingly between the restorer lines and sterile lines. Generally, important parents of hybrid rice can he identified by their particular primers. Out of the primers, 12 and 10 respectively were selected and they were polymorphic between two parents, which were adapted to examine the purity of hybrid rice seed. The seed purity of D you 527 was identified by the particular SSR primers RM337, RM244 and RM346, and it was agreeable with the result of the field test. Two combinations, D you 527 and D you 68, which rooted in the same sterile line, were distinguished by the particular SSR primer for D you 527. It showed that SSR technique has broad applicability in variety protection, authenticity and purity identification.
Chinese Journal of Rice Science