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不同NDF/NFC比的日粮对山羊小肠可吸收氨基酸组成的影响 预览 被引量:2

Effect of NDF to NFC Ratios on Absorbable Amino Acids in the Small Intestine of the Goats
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摘要 用4只体重(35±2.5)kg装瘤胃瘘管、十二指肠和回肠T型瘘管的徐淮白山羊,饲喂中性洗涤纤维/非纤维性碳水化合物比(NDF/NFC)不同的A、B、C、D 4种日粮,分别利用二氨基庚二酸和卵磷脂作为瘤胃细菌和纤毛虫的内标物测定进入十二指肠的细菌、纤毛虫及过瘤胃饲料蛋白质的各种氨基酸的流量与组成,研究对山羊小肠内可吸收氨基酸组成的影响.结果表明:小肠可吸收氨基酸(AA)中,B组细菌AA为1.54 g/d,极显著高于A组(0.45 g/d)、C组(0.36 g/d)、D组(0.39 g/d)(P<0.01),且B组占可吸收AA的38.4%,显著高于D组(24.84%)(P<0.05),极显著高于A组(13.25%)和C组(14.81%)(P<0.01);纤毛虫AA在A组为1.22 g/d,分别极显著高于B组(0.63 g/d)、C组(0.57 g/d)、D组(0.42 g/d)(P<0.01),且A组占可吸收AA的36.74%,B、C、D组分别占15.71%、23.45%、26.75%;来自过瘤胃饲料的小肠可吸收AA中,A、B组分别为1.65、1.84 g/d,显著高于C组(1.51 g/d)(P<0.05),极显著高于D组(0.76 g/d)(P<0.01),占可吸收AA%以C组(61.73%)为最高,显著高于A组(49.13%)、D组(48.41%)(P<0.05),极显著高于B组(45.88%)(P<0.01),即B组转化为微生物蛋白质的效率最高;在微生物蛋白质的AA组成中,纤毛虫的AA组成相对稳定,食糜颗粒相结合细菌的AA含量组成比食糜液相结合细菌相对稳定,食糜液相结合细菌AA变化直接影响小肠内可吸收的细菌AA组成与数量. Four Xuhuai white goats fitted with permanently ruminal cannulae, proximal duodenal and terminal ileal T-shape cannulaes were used in a 4×4 latin square design to determine the effects of four different ratios of neutral detergent fiber to N-free extract (NDF/NFC ) (NDF/NFC were 2. 75 (A), 1.71 (B), 1.12 (C) and 0. 74 (D) respectively) in diets on the absorbable amino acids(AAA) of the goats. The study used DAPA and PC respectively as internal marker of rumen bacteria and protozoa to measure the flux and composition of all the AA of bacteria and protozoa to the duodenum. The results showed that:(1)Of the AAA in the small intestine, the flow of bacterial AA in group B was 1.54g/d,which was much higher than group A(0. 45 g/d) ,C(0. 36 g/d) and D(0. 39 g/d) (P〈0. 01). The proportion of bacterial AA in group B to the total AAA was 38. 4%, which was higher than group D (24.84%) (P%0. 05),and significantly higher than group A(13.25%) and C(14. 81%)(P〈0. 01); (2)The flow of protozoal AA in group A was 1.22 g/d, significantly higher than in group B(0. 63 g/d), C(0.57 g/d) and D(0. 42 g/d)(P%0. 01), furthermore, the proportion of that in group A to the total AAA was 36. 74%, while group B, C and D was 15.71%, 23.45 % and 26.75 % respectively~ (3) Of the AAA from by-pass protein, the flow of group A,B was 1.65 g/d, 1.84 g/d, both of them were higher than C(1.51 g/d)(P%0.05) ,and significantly higher than D(0. 76 g/d)(P〈0. 01). The highest proportion to the total AAA was observed in group C(61. 37 % ), which was higher than A(49.13 % ), D(48.41% ) ( P〈0. 05), and significantly higher than B(45.88 % ) ( P〈0.01), i. e. the efficiency of dietary AA in the rumen transforming into microbial protein in group B was the greast; (4) Among the AA composition of microbial protein, the AA of protozoa was relatively stable, while the production and composition of Solid Associated Bacteria�
作者 赵国琦 贾亚红 陈小连 孙龙生 赵健亚 周为琴 郭玉华 刘墨祥 ZHAO Guo-qi, JIA Ya-hong, CHEN Xiao-lian, SUN Long-sheng, ZHAO Jian-ya, ZHOU Wei-qin, GUO Yu-hua,LIU Mo-xiang (College of Animal Science and Technology , Yangzhou University , Yangzhou 225009, China)
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2005年第12期 1292-1299,共8页 Acta Veterinaria et Zootechnica Sinica
基金 中国科学院资助项目
关键词 山羊 细菌 二氨基庚二酸 纤毛虫 卵磷脂 氨基酸 goat bacteria 2,6-diaminopimelic acid protozoa phosphatidyl choline amino acids
作者简介 赵国琦(1964-),男,吉林长春人,教授,博士,主要从事反刍动物的微生态营养及分子营养学研究。Tel:0514—7604363;E-mail:jszhaoguoqi@sohu.tom
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