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丹参酮ⅡA对MKN-45细胞生长的影响 预览 被引量:13

Effect of Tanshinone Ⅱ A on the growth of MKN-45 cell line in vitro
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摘要 目的:研究丹参酮ⅡA(TanshinoneⅡA,TanⅡA)对人胃癌细胞株MKN45的生长抑制作用.方法:选用人胃癌细胞株MKN-45,运用MTT法、流式细胞术(FCM)和逆转录聚合酶链式反应(RTPCR)半定量法检测细胞增殖、细胞周期、细胞凋亡和各组p53 mRNA表达.结果:TanⅡA可明显抑制MKN-45细胞的增殖,其抑制作用具有时间-效应和剂量-效应关系.当2.0μg/mL TanⅡA处理MKN-45细胞96 h,增殖抑制率达(74.84±0.41)%.FCM检测肿瘤细胞DNA变化,观察到TanⅡA使MKN-45细胞周期阻滞于G2/M期,出现浓度依赖性的亚'G1'峰,同时野生型p53表达增加.结论:TanⅡA能有效地抑制人胃癌细胞株MKN-45的增殖,其可能的机制与凋亡有关. OBJECTIVE: To investigate the effect of Tanshinone Ⅱ A on the growth of human gastric carcinoma cell line MKN- 45 in vitro. METHODS: Cell proliferation,cell cycle and apoptosis, the expression of p53 mRNA of cell line MKN-45 were detected by MTT assay, flow cytometry (FCM) and semiquantitative RT-PCR. RESULTS: The data showed that Tanshinone Ⅱ A effectively inhibited the proliferation of cell line MKN-45 in time- and concentrationdependent. The cell proliferation inhibition rate was (74.84 ± 0.41)% when treated with 2.0 μg/mL Tanshinone Ⅱ A for 96 hours. The change of DNA of MKN-45 cells indicated that Tanshinone Ⅱ A was able to block cell cycle progress in G2/M phase,with appearance of sub-G1 peak in a concentration- dependently. The expression of p53 was up-regulated in the process of Tanshinone Ⅱ A - inducing MKN-45 apoptosis. CONCLUSION: Tanshinone Ⅱ A can effectively inhibit the proliferation of human gastric carcinoma cell line MKN-45 in vitro and the possible mechanism may relate to modulation of the apoptosis associated gene expression, such as p53 up-regulated.
作者 董晓荣 伍钢 董继华 张涛 刘莉 侯晓华 DONG Xiao-rong , WU Gang. DONG Ji-hua , ZHANG Tao, LIU Li , HOU Xiao-hua (Union Hospital, Tongji Medical College. Huazhong University of Science and Technology , Wuhan 430022, P. R. China)
出处 《肿瘤防治杂志》 CAS 2005年第19期 1465-1468,共4页 China Journal of Cancer Prevention and Treatment
关键词 胃肿瘤/病理学 丹参酮/药理学 细胞凋亡/药物作用 蛋白质P53 stomach neoplasms/pathology tanshinone/pharmacology apoptosis/drug therapy protein p53
作者简介 董晓荣,女,硕士,主治医师,主要从事消化道肿瘤诊断和治疗的工作.Tel:86-27-65651084 E-mail:xiaorong75@163.net . 通讯作者:伍钢,男,博士,教授,主任医师,主要从事恶性肿瘤放、化疗与生物治疗的工作.Tel:8627-65650086 E-mail:wugangzr@yahoo. com. cn.
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参考文献11

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