期刊文献+

硫化氢体系在大鼠低氧性肺动脉平滑肌细胞增殖中的意义 预览 被引量:10

The Role of Hydrogen Sulfide System on Pulmonary Artery Smooth Muscle Cells Cultured of Rats in Hypoxia Condition
在线阅读 下载PDF
收藏 分享 导出
摘要 目的探讨低氧对肺动脉平滑肌细胞中胱硫醚-γ-裂解酶(CSE)基因表达的影响和硫化氢对肺动脉平滑肌细胞增殖的调节作用.方法人工培养大鼠的肺动脉平滑肌细胞,实验分为1.对照组;2.硫氢化钠(NaHS)组:10-4 mol/L NaHS处理24 h;3.低氧组:氧浓度1%以下24 h;4.低氧+NaHS组:低氧24 h,10-4 mol/L NaHS.每组各6孔细胞.用3H-TdR掺入估计细胞增殖,用定量竞争性RT-PCR方法测定CSE mRNA含量,评价CSE基因表达.结果低氧组平滑肌细胞中的CSE mRNA含量较对照组减少67%(P<0.01).低氧组细胞的3H-TdR掺入较对照组增加66%(P<0.01);NaHS组细胞的3H-TdR掺入较对照组减少37%(P<0.01);低氧+NaHS组的3H-TdR掺入较低氧组减少29%(P<0.01).结论低氧使人工培养的大鼠肺动脉平滑肌细胞中CSE基因表达下调,硫化氢对大鼠肺动脉平滑肌细胞增殖有抑制作用. Objective To explore the influence of hypoxia on cystathionine gamma lyase (CSE) gene expr ession of rat cultured pulmonary artery smooth muscle cells (PASMCs) and the mod ulatory effect of hydrogen sulfide on PASMCs proliferation.Methods Rat′s cultured PASMCs were randomly divided into contr ol group,NaHS group,hypoxia group and hypoxia +NaHS group. Each group contains 6 wells of cells. Hypoxic challenge was performed with oxygen concentr ation≤1%. The cell proliferation rate was measured by 3H-thymidine i ncorporation into DNA of the PASMCs. The expression of CSE mRNA was measured by quantitative competitive reverse-transcription polymerase chain reaction (RT-PCR ).Results For the PASMCs in hypoxia group, the amount of CSE mRN A was down-regulated about 67% (P<0.01) compared with control group. Compare d with control group, the 3H-thymidine incorporation was increased by 66% (P<0.01) for PASMCs in hypoxia group; compared with control group, the 3H-thymidine incorporation was decreased by 37% for PASMCs in NaHS gr oup; compared with hypoxia group,the 3H-thymidine incorporation was d ecreased by 29% for PASMCs in hypoxia+NaHS group.Conclusion Hypoxia challenge can down-regulate the CSE gene exp ression;and hydrogen sulfide can suppress the proliferation of rat′s PASMCs. J Appl Clin Pediatr,2005,20(2):140-141
作者 张春雨 丛柏林 宫丽敏 杜军保 ZHANG Chun-yu1,CONG Bo-lin2,GONG Li-min1,DU Jun-bao1 (1.Department of Pediatrics,the First Hospital of Peking University,Beijing 1000 34,China;2.Chengde Medical College,Chengde 067000,China)
出处 《实用儿科临床杂志》 CAS CSCD 北大核心 2005年第2期 140-141,i002,共3页 Journal of Applied Clinical Pediatrics
基金 国家自然科学基金,国家重点基础研究发展计划(973计划),高等学校博士学科点专项科研项目
关键词 硫化氢 低氧 肺动脉平滑肌细胞 hydrogen sulfide hypoxia pulmonary artery smooth mus cle cells
  • 相关文献

参考文献10

二级参考文献29

  • 1石湘芸,张昭馥.高血压与内皮素的临床与实验研究[J].海军总医院院刊,1993,6(2):73-76. 被引量:4
  • 2汪浩川,刘秉文.人动脉平滑肌细胞贴块培养法[J].华西医科大学学报,1995,26(1):105-108. 被引量:34
  • 3薛全福,谢剑鸣.常压缺氧性大鼠肺动脉高压模型的建立[J].中华结核和呼吸杂志,1989,12(6):350-352. 被引量:152
  • 4孙波 刘文利.右心导管测定大鼠肺动脉压的实验方法[J].中国医学科学院学报,1984,6:465-465. 被引量:125
  • 5Hosok R, Matsuki N, Kimura H.The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide [J]. Biochem Biophys Res Commun, 1997,237(3) : 527 - 531. 被引量:1
  • 6Hirata Y, Taragi Y, Takata S, et al. CGRP receptor in cultured vascular smooth muscle and endothelial cells[ J ].Biochem Biophys Res Commun, 1988, 151(3):1113- 1118. 被引量:1
  • 7Zhao W, Zhang J, Lu Y, et al. The vasorela.xant effect of H2S as a novel endogenous gaseous K (ATP) channel opener[J]. EMBO J, 2001, 20 (21): 6008-6016. 被引量:1
  • 8Li TC(李田昌),Pang YZ(庞永正),Su JY(苏静怡) ,et al.A method of mitogen - aetivited protein kinase, activity assay[J]. Bas Med Sci Clin, 1999,16 (2):158- 160. 被引量:1
  • 9Bogoyevitch MA, Glennon PE, Andersson MB, et al. En-dothelin - 1 and fibroblast growth factors stimulate the mitogen- activited protein kinase signaling cascade in cardiac myocytes[J]. J Biol Chem, 1994, 269 (2): 1110- 1119. 被引量:1
  • 10Safar ME, London GM,Asmar R, et al. Recent Advances on Large Arteries in Hypertension[J]. Hypertension, 1998,32:156-161 被引量:1

共引文献133

同被引文献158

引证文献10

二级引证文献57

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈