目的:了解呼吸道感染住院病人流感嗜血杆菌、副流感嗜血杆菌及肺炎链球菌的感染和耐药性以及流感嗜血杆菌和副流感嗜血杆菌β-内酰胺酶的产生率.方法:收集呼吸道感染患者的痰标本共1263份,按细菌学常规方法进行分离培养、鉴定和药敏试验,以Ni trocefin 纸片法测定β-内酰胺酶的产生率.结果:1263份痰标本中共分离出流感嗜血杆菌139株,副流感嗜血杆菌85株,肺炎链球菌228株.139株流感嗜血杆菌产β-内酰胺酶51株(36.69%),85株副流感嗜血杆菌产β-内酰胺酶32株(37.65%).139株流感嗜血杆菌对氨苄西林、四环素、SMZ 高度耐药,对利福平和阿奇霉素的耐药率为0.00%.85株副流感嗜血杆菌对氨苄西林、四环素和氧氟沙星耐药严重,对利福平的耐药率为0.00%.228株肺炎链球菌对红霉素、克林霉素、四环素、SMZ 耐药率相当高,对青霉素中介率高达23.25% .结论:呼吸道苛养菌对多种抗生素具有较明显的耐药性,临床微生物室应加强对呼吸道标本中苛养菌的常规分离培养及药敏试验,指导临床医生合理使用抗生素以延缓耐药率的快速上升.
Objectives:To investigate drug-resistance and infection status of fastidious bacteria in low respiratory tract and enzyme-producing rate of Haemophilus.Methods:1263 specimens of patients with respiratory tract infection were collected, and specimens were tested according to the routine methods.β-lactamase was measured by nitrocefin paper method. Results:Among 1263 samples from low respiratory tract, 139 strains of Haemophilus influenzae, 85 strains of Haemophilus parainfluenzae and 228 strains of Streptcoccus pneumoniae were found. The positive rate of β-lactamase was 36.69%(51/139) for Haemophilus influenzae and 37.65%(32/85) for Haemophilus parainfluenzae. The drug resistance rate of Haemophilus influenzae was extremely high in ampicillin,tetracycline and SMZ. No resistant strains to rifampicine and azithrymocin were detected. The percentage of resistance of Haemophilus parainfluenzae was serious in ampicillin,tetracycline and ofloxacin .No resistant strains to rifampicine were detected. Streptcoccus pneumoniae has emerged highly resistant to erythromycine, clindamycine, tetracycline and SMZ. The rate of intermediate resistance to penicillin was 23.25%. Conclusions:The fastidious bacteria of low respiratory tract have significantly resistance to many kinds of antibiotics, so the correct use of antibiotics is the best way to decrease drug- resistance.
Journal of Sichuan Continuing Education College of Medical Sciences