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急性呼吸综合征患者外周血树突状细胞表型和功能的变化

The alteration of phenotype and function of peripheral dendritic cells in severe acute respiratory syndrome
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摘要 目的探讨严重急性呼吸综合征病毒(SARSV)感染对树突状细胞(DC)表型和功能的影响.方法采用流式细胞仪检测SARS发病期患者(9例)和正常人(7例)DC的表型;分离培养DC和T淋巴细胞,用混合淋巴细胞反应检测SARS患者和正常人DC对T淋巴细胞增殖的诱导作用.结果 SARSV感染抑制了DC类刺激分子CD80、CD86和HLAⅡ类分子HLA-DR以及与DC成熟有关的分子CD83的表达;抑制DC诱导T淋巴细胞增殖的能力.结论 SARSV可抑制DC的分化成熟和抗原递能力. Objective To explore the pathogenesis of severe acute respiratony syndrome(SARS)Methods The changes of phenotypes of dendritic cells (DCs) were assessed with flow cytometry in 9 SARS patients and 7 healthy persons.The DCs and T-lymphocytes were isolated with gradient centrifugation and cultured in vitro. The proliferation inductions of DCs to T-lymphocytes were measured with mixed lymphocyte reaction.Results Severe acute respiratory syndrome virus suppressed the expression of costimulatory molecules (CD80, CD86), HLA class Ⅱ(HLA-DR) and maturation associated molecule (CD83) on DCs,with significant difference between the two groups,P<0.05. SARS virus inhibited the proliferation function of DC on T-lymphocytes (P<0.05).Conclusion SARS virus can suppress the differentiation and maturation,and the antigen presentation functions of DCs.
作者 刘树人 张雁 陈汉生 谢庆 曾水娣 王添章 李灼亮 孔祥平 LIU Shuren, ZHANG Yan, CHEN Hansheng,et al. Department of Infectious Disease, Infectious Disease Center of PLA, The 458 Hospital of PLA, Guangzhou 510602, China.
机构地区 510600
出处 《中华急诊医学杂志》 CAS CSCD 2004年第9期 616-618,共3页 Chinese Journal of Emergency Medicine
关键词 DC 表型 患者 T淋巴细胞增殖 外周血树突状细胞 正常人 急性呼吸综合征 成熟 发病期 分离培养 Severe acute respiratory syndrome Dendritic cells Phenotypes Function
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